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¥329Polyethylenimine,Linear,Mw 25,000
线性化聚乙烯亚胺(PEI 25,000)
基本描述:
线性化聚乙烯亚胺PEI 25,000,一种高电荷阳离子聚合物,非常容易结合于高电荷阴离子基质。工业应用上,线性化PEI可改善负电荷染料的物理外观,以调整染料的化学特性和增强染料与固相基质的黏附能力,常用作黏附增强剂,作用同多聚赖氨酸(poly-lysine);科研应用上,线性化PEI能与DNA或其他负电荷生物大分子简单结合,正是这一特性,使得成为一种非常行之有效的载体运输介质(Vector carrier),即转染试剂。
化学性质:
CAS NO. | 9002-98-6, 26913-06-4 |
Formula | (CH2CH2NH)n |
Molecular Weight | 25,000 |
Melting Point | 73-75º |
Solubility | Soluble In: hot water, cold water at low pH, methanol, and ethanol. Insoluble In: benzene, ethyl ether and acetone |
Appearance | White to yellow solid |
订购信息:【*,现货供应】
品牌 | 货号 | 名称 | 规格 | *(元) |
Polysciences | 23966-100 | 线性聚乙烯亚胺(MW 25,000) | 100mg | 2310 |
另,提供更高转化效率的PEI Max 40,000,自由基形式分子量相当于PEI 25,000,研究证实其体外质粒DNA转染效率提高达21倍。 | ||||
Polysciences | 24765-100 | 线性聚乙烯亚胺盐酸盐Max,(MW 40,000) (Equivalent to Mw 25,000 in Free Base Form) | 100mg | 2502 |
使用方法【来源于:CSH protocols,仅作交流学习】 :
1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.
2. Add 500 mg of PEI to the beaker with stirring.
3. Add concentrated HCl drop-wise to the solution to pH<2.0.
4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH<2.0 throughout.
Approximay 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.
5. Add concentrated NaOH drop-wise to the solution to pH 7.0.
Approximay 500 μL of 10 M NaOH will be required to neutralize the PEI solution.
6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.
7. Filter-sterilize the solution through a 0.22-μm membrane.
8. Store aliquots of the desired volume at -20°C.
应用文献【基因转染用途】 :
Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.
——Written/Edited by V. Shallan【版权归MKBio懋康所有】
线性化聚乙烯亚胺PEI 25000细胞传代
线性化聚乙烯亚胺PEI 25000细胞传代