Anti--CD30/TNFRSF8/FITC

Catalog Number : RSF-2495R-FITC

Quantity size : 100ug （2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. ）

Background: CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L （CD153）. The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas （including Burkitt's lymphomas）, virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor..

Specificity : · Anti- -CD30/TNFRSF8 is a rat monoclonal antibody, /FITC

           · specific for -CD30/TNFRSF8 of hu

           · Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG 

· mol wt: 63kDa

Application :  Flow-Cyt=1:50-200 

 IF=1:50-200

            · Immunohistochemistry : 1:20-1:200

            · Immunocytochemistry: 1:20-1:200

· Excitation spectrum: 490-495nm

· Emission spectrum: 520-530nm

. KLH conjugated synthetic peptide derived from mouse TNFRSF  

· Optimal working dilutions must be determined by the end user.

· protect from light.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.