Anti-E-cadherin/FITC

Catalog Number : RSF-1016R-FITC

Quantity size : 100ug (2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 10mg/ml BSA and 0.1% Sodium azide. )

Relevance: Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as ∫-catenin, to regulate cadherin function.

Specificity : · anti-E-cadherin is a mouse monoclonal antibody, /FITC

           · specific for E-cadherin  of  human  

           · Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG 

· mol wt: 80kDa

Application : · FCM: 1:20-100

.IF=1:50-200

   .ICC=1:50-200

            · Immunohistochemistry : 1:20-1:200

            · Immunocytochemistry: 1:20-1:200

· Excitation spectrum: 490-495nm

· Emission spectrum: 520-530nm

.KLH conjugated synthetic peptide derived from human CK18 C-terminus

· Optimal working dilutions must be determined by the end user.

· protect from light.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.