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Blood Group H Disaccharide 血型H二糖
¥2180伊文思蓝染色液 指示剂
¥395TRITC标记葡聚糖(2000 kDa)
¥1828TRITC-Dextran 标记葡聚糖(150 kDa)
¥1828FITC-Dextran FITC标记葡聚糖(150 kDa)
¥948黄/蓝色溶酶体荧光探针生物试剂
¥583DND-189 绿色溶酶体荧光探针生物试剂
¥583N-羟基琥珀酰亚胺磺酸钠盐 免疫学
¥753Trypan Blue 台盼蓝生物试剂
¥125Gramicidin 短杆菌肽 多肽抗生素
¥298Zeocin (Powder) 抗生素
¥360全能核酸酶(不含标签)蛋白质研究
面议Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素/硫酸诺尔丝菌素
(分子遗传学优越的筛选性抗生素)
搜索关键词:
链丝菌素 Streptothricin; clonNAT; 选择性抗生素;革兰氏阳性和阴性菌; 酵母菌和丝状真菌;植物; sat1基因; nat1基因;CAS:96736-11-7
诺尔斯菌素(Nourseothricin,缩写NTC),英文同义名Streptothricin和clonNAT,由诺尔斯氏链霉菌( Streptomyces noursei)代谢产生的一种广谱型抗生素,因结构上具链丝菌素(Streptothricin,STs)化学基团,故归属于此类抗生素亚家族。天然产物提取的诺尔斯菌素(Nourseothricin)一般为ST-F和ST-D的混合物,具有极其宽广的抑制生长活性,原核生物如细菌;真核生物如包括白色念球菌在内的各种酵母菌、丝状真菌、原生生物、昆虫和植物【见下表格1】。诺尔斯菌素的作用机制是通过诱导mRNA错误编码,来抑制蛋白合成。
基于以上特性,诺尔斯菌素(Nourseothricin)是基因重组工程研究中非常优秀的选择性抗生素。抗性基因nat1zui初从S. noursei分离所得,此基因能编码表达诺尔斯菌素N-乙酰转移酶,通过对诺尔斯菌素(NTC)上与糖基部分相连的ß-赖氨酸上的ß-氨基基团进行单乙酰化修饰,使得抗生素失活。研究发现,nat1基因在多种异质体系内发挥作用,使其在分子遗传学中是非常有价值的选择性工具。后面陆续发现具相同抗性机理的基因有:来自大肠杆菌E. Coli的基因stat1,stat2,stat3;来自链霉菌S. lavendulae的stat基因。另外,还有来自链霉菌S. albulus的新型sttH基因,作用机制不同,抗性在于编码表达异分支酸类型水解酶,能打开streptolidine内酰胺环上的酰胺键,将其转化成无活性的酸性产物。sttH基因抗性似乎只对酵母菌有活性,对细菌无效。
Nourseothricin Sulfate(NTC) 硫酸诺尔斯菌素/诺尔丝菌素 生化试剂 具有以下应用优势:
1. 粉末或者液体的稳定性非常高。粉末+4℃保存10年有效,+20℃保存2年有效。
2. 粉末具非常高的水溶性(1g/L);
3. 具非常低或几乎无背景抗性:因抗性蛋白仅在细胞内表达,不会在细胞培养基内被降解。
4. 不会与大量其他抗生素,如氨基糖苷类抗生素潮霉素B或遗传霉素G418发生交叉反应。
5. 不能用作诊疗抗生素,对临床上治疗的细菌微生物呈现出极弱抗性。因此与诊疗抗生素交叉反应很小。
6. 特别适用于酵母分子生物学研究。不像一些营养缺陷型选择性基因,诺尔斯菌素抗性不会影响酵母正常生长,能用于工程菌,或者缺少营养性筛选基因表达的野生菌。
Nourseothricin Sulfate(NTC) 硫酸诺尔斯菌素/诺尔丝菌素 生化试剂使用方法和推荐浓度:
储存液配制:见懋康内容
建议筛选浓度:诺尔斯菌素在相对较低的浓度下即可有效用于重组工程菌的筛选,大多数有机体的筛选浓度多在50-200µg/ml区间内。具体可参考下表1。
货号 | 产品名称 | 规格 | 价格(元) | 货期 |
MS0026-20MG | Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素 | 20mg | 396 | 现货 |
MS0026-100MG | Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素 | 100mg | 1056 | 现货 |
MS0026-500MG | Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素 | 500mg | 4186 | 现 |
MS0026-1000MG | Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌 | 1g | 7146 | 现货
|
另提供美国Cayman公司,*的Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素,CAS:96736-11-7,货期:3-4周
货号 | 产品名称 | 规格 | 价格(元) | 品牌 |
16227 | Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素 | 1mg | 350 | Cayman |
16227 | Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素 | 5mg | 700 | Cayman |
16227 | Nourseothricin (NTC) Sulfate 硫酸诺尔斯菌素 | 10mg | 1232 | Cayman |
相关产品:
货号 | 产品名称 | 规格 | 价格(元) | 货期 |
MS0007-1ML | Blasticidin S (10 mg/ml) 杀稻瘟菌素S (灭瘟素)(10 mg/ml) | 1ml | 450 | 现货 |
MS0007-5ML | 5×1ml | 2050 | 现货 | |
MS0007-10ML | 10×1ml | 3600 | 现货 | |
MS0009-125MG | Zeocin (100mg/ml) 盐酸博莱*/酶素(100 mg/ml) | 125mg | 420 | 现货 |
MS0009-1000MG | Zeocin (100mg/ml) 盐酸博莱*-酶素(100 mg/ml) | 8×125mg | 2850 | 现货 |
MS0010-1G | G418 Sulfate (Geneticin) 遗传霉素 | 1g | 400 | 现货 |
MS0010-5G | G418 Sulfate (Geneticin) 遗传霉素 | 5g | 1600 | 现货 |
MS0011-25MG | Puromycin Dihydrochloride 嘌呤霉素盐酸盐 | 25mg | 540 | 现货 |
MS0011-100MG | Puromycin Dihydrochloride 嘌呤霉素盐酸盐 | 100mg | 1800 | 现货 |
MS0011-500MG | Puromycin Dihydrochloride 嘌呤霉素盐酸盐 | 500mg | 8500 | 现货 |
表1 zui低抑制浓度和选择性浓度列表【详见公司MKbio】
参考文献:
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— —Written/Edited by V. Shallan【版权归MKBio懋康所有】