Alamar Blue(阿尔玛蓝,阿尔玛兰)LD50测定方法
时间:2018-04-07 阅读:1505
。。前言:
Alamar Blue(阿尔玛蓝,阿尔玛兰)是目前的细胞增殖和细胞毒性检测试剂之一,以其低毒性安全,操作灵活简单,灵敏度高,适用细胞范围广等优点被越来越多的科研工作者所认可。我司(懋康生物)已成功推出本热度单品并被市场认可,现结合大家关心的问题,一 一列出,以作交流学习用。
。。概念:
阿尔玛蓝(Alamar Blue)是一种细胞活力检测试剂,包含一种具细胞膜渗透性、无毒性且弱蓝色荧光的指示剂,即刃天青(resazurin)。刃天青(resazurin)是一种氧化还原(REDOX)指示剂,根据细胞代谢还原情况发生颜色变化。氧化态的刃天青呈紫蓝色且基本无荧光,其还原态产物试卤灵(resorufin)转变为粉红色且发荧光。
。。本期话题:
alamarBlue® protocol: Method to determine LD50 using alamarBlue
alamarBlue can be used to determine the median lethal dose LD50 value and similar assays testing the effects of a toxin, radiation, or pathogen against cell cultures.
Percentage difference in reduction (equation 5) | = | A LW - ( A HW x R O ) for test well A LW - ( A HW x R O ) for control well | x100
| |
Use semi-log graph paper to plot the percent of untreated control (using equation 5) for each dilution of a given test agent on the y-axis vs. the concentration of the test agent on the x-axis.
To determine the LD50 endpoint from the graph, read from where the 50 percent point intercepts the Dose.
Response Curve to the concentration along the x-axis. This concentration is the LD50 value.
订购信息:原料来自持有生厂商,高品质产品,现货供应,质量保证,已有大量的忠实粉,咨询,,。
产品名称 | 产品编号 | 规格 | 价格(元) | 货期 |
Alamar Blue 阿尔玛蓝细胞增殖及毒性检测试剂 | MX3006-5ML | 5ml (500T) | 295 | 现货 |
Alamar Blue 阿尔玛蓝细胞增殖及毒性检测试剂 | MX3006-10ML | 10ml (1000T) | 550 | 现货 |
Alamar Blue 阿尔玛蓝细胞增殖及毒性检测试剂 | MX3006-50ML | 50ml (5000T) | 1925 | 现货 |
【产品的使用方法见说明书】。