ELISAThe following video tutorial described how to run a sandwich ELISA, also known as enzyme-linked immunosorbent assay, using a general PeproTech protocol.

       接下来的实验指导讲述PeproTech夹心法ELISA，即酶联免疫吸附试验的通用步骤

The capture, standard, and detection are supplied as stable lyophilized products, and should be stored at -20℃ until ready for use.
       捕获抗体、标准品和检测抗体均为冻干粉，使用前应保存于-20℃

Reconstituted Capture, Standard, and Detection components are only guaranteed to be stable for up to 2 weeks when stored at 4 ℃.
       捕获抗体、标准品和检测抗体重悬后，在4℃时zui长保存2周。

If you have reconstituted the EDK, and plan on using it for a duration greater than two weeks, aliquot and store at -20℃ for up to 6 months.
       如果您已重悬了EDK的各组份，并准备在2周之后使用，请将重悬的组分分装并冻存于-20℃，zui长可保存6个月。

In contrast to the other three EDK components, the Avidin-HRP vial comes ready to use
       与EDK的其它三个组分不同，Avidin-HRP为即用型

In order to avoid harmful repeated freeze/thaw cycles, or long-term storage at 4 ℃ w

hich mean advisory functionality. Aliquot the Avidin-HRP into ten 6uL vials upon receipt and stored at -20 ℃ are stable for up 2 years from the date of receipt.
       目的是避免反复冻融或长期4℃保存对该组分的功能损害。收到Avidin-HRP后，立即将其分装为6ul/管，共10管，冻存于-20℃，zui长可保存2年