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广州市所在地
西班牙Certest乙型流感病毒重组核蛋白
广州健仑生物科技有限公司
广州健仑长期供应各种生物原料,主要代理品牌:西班牙Certest。
Certest Biotec,S.L. 是一家独立的生物技术公司,致力于人类临床领域的IVD诊断产品的开发和制造。快速检测是基于快速、准确和易于操作的诊断产品。 此外,Cerstest还有多种实时PCR产品,可以用于医院临床、实验室科研等。
Certest公司于2002年在萨拉戈萨成立,是一家创新技术型公司。公司的发展是基于新产品的开发、市场空间和机遇的探索,十几年来一直以高度专业化,以客户为导向,不断得创新,优化产品,专业知识,取得广大客户的信任和支持。Certest的质量体系已通过ISO 13485认证。
主要产品包括各种生物单克隆抗原抗体、重组蛋白。
轮状病毒单克隆抗体、腺病毒抗体、星状病毒单克隆抗体、诺如病毒单克隆抗体、幽门螺旋杆菌抗体、隐球菌抗体、肠道病毒抗体、贾第鞭毛虫抗体、弯曲杆菌抗体、阿米巴原虫抗体、呼吸道合胞病毒单抗等等。
西班牙Certest乙型流感病毒重组核蛋白
我司还提供其它进口或国产试剂盒:登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。
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西班牙Certest公司简介
Certest Biotec, S.L. is an independent biotechnology company devoted to the development and manufacturing of IVD diagnostic products in human clinical field. Our Rapid Test are based in a fast, reliable and easy-interpretation process. In addition, VIASURE contains a wide range of products for Real Time PCR that allow to identify the causal pathogens of the infectious diseases in physicians’ offices, labs and hospitals.
The company was established in Zaragoza in 2002 as an innovative and technology-based company. The company’s growth is based on the development of new products and the exploration for new market niches and opportunities. The key to understand its success is the way to understand its organization: highly specialised and customer-oriented based.
Mission, Vision, Values and Human Team
Mission
To develop, produce and market a wide range of innovative solutions and quality in vitro diagnostics for the detection of viruses, bacteria and parasites.
Vision
Being the international leader company in research and development for in vitro diagnostic solutions, increasing our distribution network through added value creation and company-customer trust.
Values
Customer orientation · Ethical behaviour · Teamwork · Continuous research and innovation ·Trained and motivated team · Quality and creativity in all processes
Human team
The multi-skilled team is a dedicated and experience professional group responsible for dealing with all the different company activities in an international environment.
Commitment to Innovation, Export and High-Technology.
Certest’s Quality System has been certified with ISO 13485.
One of the main objectives of Certest is to achieve customer satisfaction. For this, the management of the company ensures that customer requirements are determined and reviewed, identifying their needs and expectations, and translating them into requirements, all in order to maintain customer satisfaction.
See/Download the Certest Biotec ISO Standard Certification Certest Policy is consistent with its primary purpose, that of getting to all its products and services, quality and reliability, meeting the requirements and maintaining the effectiveness of the management system.
“Comparative study of two diagnostic tests for detection of RNA Zika Virus (ZIKV) in clinical samples”
“Comparison of Real-Time PCR test with the routine diagnosis technique to detect enteric pathogenic protozoa”
“Diagnostic of the Enteric Virus infection by Real-Time PCR in stool samples”
“Clinical performance evaluation of lyophilized VIASURE Real Time PCR Detection Kits for pathogen detection by participation in External Quality Assessment (EQA) programs”
“Detection and differentiation of Influenza A Virus, Influenza B Virus and H1N1 (pdm09) variant through an stabilized Real Time PCR Mix”
“Comparison of different molecular methods for the detection of non-influenza respiratory virus”virus”
【西班牙Certest生物原料】
货号 | 产品名称 | 规格 | 英文名称 |
MT-16R15 | 轮状病毒单克隆抗体(克隆R15) | x1mg | Anti-Rotavirus Mab (clone R15) |
MT-16R15s1 | 轮状病毒单克隆抗体(克隆R15) | x100μg | Anti-Rotavirus Mab (clone R15) |
MT-18A15 | 腺病毒抗体(克隆A15) | x1mg | Anti-Adenovirus Mab (clone A15) |
MT-18A15s1 | 腺病毒抗体(克隆A15) | x100μg | Anti-Adenovirus Mab (clone A15) |
MT-18AT18 | 星状病毒单克隆抗体(克隆AT18) | x1mg | Anti-Astrovirus Mab (clone AT18) |
MT-18AT18s1 | 星状病毒单克隆抗体(克隆AT18) | x100μg | Anti-Astrovirus Mab (clone AT18) |
MT-18AT8 | 星状病毒单克隆抗体(克隆AT8) | x1mg | Anti-Astrovirus Mab (clone AT8) |
MT-18AT8s1 | 星状病毒单克隆抗体(克隆AT8) | x100μg | Anti-Astrovirus Mab (clone AT8) |
MT-18NG28 | 诺如病毒GI单克隆抗体(克隆NG28) | x1mg | Anti-Norovirus GI Mab (clone NG28) |
MT-18NG28sl | 诺如病毒GI单克隆抗体(克隆NG28) | x100μg | Anti-Norovirus GI Mab (clone NG28) |
MT-18NP8 | 诺如病毒GII单克隆抗体(克隆NP8) | x1mg | Anti-Norovirus GII Mab (clone NP8) |
MT-18NP8sl | 诺如病毒GII单克隆抗体(克隆NP8) | x100μg | Anti-Norovirus GII Mab (clone NP8) |
MT-18EV5 | 肠道病毒抗体(克隆EV5) | x1mg | Anti-Enterovirus Mab (clone EV5) |
MT-18EV5sl | 肠道病毒抗体(克隆EV5) | x100μg | Anti-Enterovirus Mab (clone EV5) |
MT-16P2 | 幽门螺旋杆菌抗体(克隆P2)HP抗体 | x1mg | Anti-H. pylori Mab (clone P2) |
MT-16P2sl | 幽门螺旋杆菌抗体(克隆P2)HP抗体 | x100μg | Anti-H. pylori Mab (clone P2) |
MT-16GD10 | 艰难梭菌抗体(克隆GD10) | x1mg | Anti-GDH Mab (clone GD10) |
MT-16GD10sl | 艰难梭菌抗体(克隆GD10) | x100μg | Anti-GDH Mab (clone GD10) |
MT-18TA5 | 艰难梭菌毒素A抗(克隆TA5) | x1mg | Anti-CD Toxin A Mab (clone TA5) |
MT-18TA5sl | 艰难梭菌毒素A抗(克隆TA5) | x100μg | Anti-CD Toxin A Mab (clone TA5) |
MT-18TA7 | 艰难梭菌毒素A抗(克隆TA7) | x1mg | Anti-CD Toxin A Mab (clone TA7) |
MT-18TA7sl | 艰难梭菌毒素A抗(克隆TA7) | x100μg | Anti-CD Toxin A Mab (clone TA7) |
MT-18TB41 | 艰难梭菌毒素B抗(克隆TB41) | x1mg | Anti-CD Toxin B Mab (clone TB41) |
MT-18TB41sl | 艰难梭菌毒素B抗(克隆TB41) | x100μg | Anti-CD Toxin B Mab (clone TB41) |
MT-18TB48 | 艰难梭菌毒素B抗(克隆TB48) | x1mg | Anti-CD Toxin B Mab (clone TB48) |
MT-18TB48sl | 艰难梭菌毒素B抗(克隆TB48) | x100μg | Anti-CD Toxin B Mab (clone TB48) |
MT-18E10 | 大肠杆菌O157抗体(克隆E10) | x1mg | Anti-E. coli O157 Mab (clone E10) |
MT-18E10sl | 大肠杆菌O157抗体(克隆E10) | x100μg | Anti-E. coli O157 Mab (clone E10) |
MT-16CA29 | 弯曲杆菌抗体(克隆ECA29) | x1mg | Anti-Campylobacter Mab (clone CA29) |
MT-16CA29sl | 弯曲杆菌抗体(克隆ECA29) | x100μg | Anti-Campylobacter Mab (clone CA29) |
MT-18K31 | 隐球菌抗体(克隆K31) | x1mg | Anti-Crypto Mab (clone K31) |
MT-18K31sl | 隐球菌抗体(克隆K31) | x100μg | Anti-Crypto Mab (clone K31) |
MT-16G18 | 贾第鞭毛虫抗体(克隆G18) | x1mg | Anti-Giardia Mab trophozoite protein (clone G18) |
MT-16G18sl | 贾第鞭毛虫抗体(克隆G18) | x100μg | Anti-Giardia Mab trophozoite protein (clone G18) |
MT-16G22 | 贾第鞭毛虫抗体(克隆G22) | x1mg | Anti-Giardia Mab trophozoite protein (clone G22) |
MT-16G22sl | 贾第鞭毛虫抗体(克隆G22) | x100μg | Anti-Giardia Mab trophozoite protein (clone G22) |
MT-18EH30 | 阿米巴原虫抗体(克隆H30) | x1mg | Anti-Entamoeba Mab (clone EH30) |
MT-18EH30sl | 阿米巴原虫抗体(克隆H30) | x100μg | Anti-Entamoeba Mab (clone EH30) |
MT-16CP14 | 钙结合蛋白单克隆抗体(克隆CP14) | x1mg | Anti-Calprotectin Mab (clone CP14) |
MT-16CP14sl | 钙结合蛋白单克隆抗体(克隆CP14) | x100μg | Anti-Calprotectin Mab (clone CP14) |
MT-16F22 | 血红蛋白单抗(克隆F22) | x1mg | Anti-Haemoglobin Mab (clone F22) |
MT-16F22sl | 血红蛋白单抗(克隆F22) | x100μg | Anti-Haemoglobin Mab (clone F22) |
MT-16LC16 | 乳铁蛋白单抗(克隆LC16) | x1mg | Anti-Lactoferrin Mab (clone LC16) |
MT-16LC16sl | 乳铁蛋白单抗(克隆LC16) | x100μg | Anti-Lactoferrin Mab (clone LC16) |
MT-16LC4 | 乳铁蛋白单抗(克隆LC4) | x1mg | Anti-Lactoferrin Mab (clone LC4) |
MT-16LC4sl | 乳铁蛋白单抗(克隆LC4) | x100μg | Anti-Lactoferrin Mab (clone LC4) |
MT-26VP6 | 轮状病毒VP6重组蛋白 | x1mg | Rotavirus VP6 recombinant protein |
MT-26VP6 | 轮状病毒VP6重组蛋白 | x100μg | Rotavirus VP6 recombinant protein |
MT-25HEX | 腺病毒HEXON重组蛋白 | x1mg | Adenovirus HEXON recombinant protein |
MT-25HEXsl | 腺病毒HEXON重组蛋白 | x100μg | Adenovirus HEXON recombinant protein |
MT-25AST | 星状病毒衣壳重组蛋白 | x1mg | Astrovirus capsid recombinant protein |
MT-25ASTsl | 星状病毒衣壳重组蛋白 | x100μg | Astrovirus capsid recombinant protein |
MT-25NGI1 | 诺如病毒GI.1重组P结构域 | x1mg | Norovirus GI.1 recombinant P domain |
MT-25NGI1sl | 诺如病毒GI.1重组P结构域 | x100μg | Norovirus GI.1 recombinant P domain |
MT-25NGI3 | 诺如病毒GI.3重组P结构域 | x1mg | Norovirus GI.3 recombinant P domain |
MT-25NGI3sl | 诺如病毒GI.3重组P结构域 | x100μg | Norovirus GI.3 recombinant P domain |
MT-25NGII10 | 诺如病毒GII.10重组P结构域 | x1mg | Norovirus GII.10 recombinant P domain |
MT-25NGII10sl | 诺如病毒GII.10重组P结构域 | x100μg | Norovirus GII.10 recombinant P domain |
MT-25NGII17 | 诺如病毒GII.17重组P结构域 | x1mg | Norovirus GII.17 recombinant P domain |
MT-25NGII17sl | 诺如病毒GII.17重组P结构域 | x100μg | Norovirus GII.17 recombinant P domain |
MT-25NGII14 | 诺如病毒GII.4重组P结构域 | x1mg | Norovirus GII.4 recombinant P domain |
MT-25NGII14sl | 诺如病毒GII.4重组P结构域 | x100μg | Norovirus GII.4 recombinant P domain |
MT-25ETV | 肠道病毒VP1重组蛋白 | x1mg | Enterovirus VP1 recombinant protein |
MT-25ETVs1 | 肠道病毒VP1重组蛋白 | x100μg | Enterovirus VP1 recombinant protein |
MT-25PCH | 幽门螺杆菌重组外膜蛋白 | x1mg | H. pylori recombinant outer membrane protein |
MT-25PCHs1 | 幽门螺杆菌重组外膜蛋白 | x100μg | H. pylori recombinant outer membrane protein |
MT-25GDH | 艰难梭菌GDH重组蛋白 | x1mg | Clostridium difficile GDH recombinant protein |
MT-25GDHsl | 艰难梭菌GDH重组蛋白 | x100μg | Clostridium difficile GDH recombinant protein |
MT-24TXA | 艰难梭菌毒素A重组蛋白(无毒性片段) | x1mg | C. difficile Toxin A recombinant protein (fragment without toxic activity) |
MT-24TXAsl | 艰难梭菌毒素A重组蛋白(无毒性片段) | x100μg | C. difficile Toxin A recombinant protein (fragment without toxic activity) |
MT-24TXB | 艰难梭菌毒素B重组蛋白(无毒性片段) | x1mg | C. difficile Toxin B recombinant protein (fragment without toxic activity) |
MT-24TXBsl | 艰难梭菌毒素B重组蛋白(无毒性片段) | x100μg | C. difficile Toxin B recombinant protein (fragment without toxic activity) |
MT-25STX | 大肠杆菌O157 VT1重组蛋白 | x1mg | E. coli O157 VT1 recombinant protein |
MT-25STXsl | 大肠杆菌O157 VT1重组蛋白 | x100μg | E. coli O157 VT1 recombinant protein |
MT-25VT2 | 大肠杆菌O157 VT2重组蛋白 | x1mg | E. coli O157 VT2 recombinant protein |
MT-25VT2sl | 大肠杆菌O157 VT2重组蛋白 | x100μg | E. coli O157 VT2 recombinant protein |
MT-25CCP | 弯曲杆菌重组外膜蛋白 | x1mg | Campylobacter coli recombinant outer membrane protein |
MT-25CCPsl | 弯曲杆菌重组外膜蛋白 | x100μg | Campylobacter coli recombinant outer membrane protein |
MT-25CEP | 空肠弯曲杆菌重组外膜蛋白 | x1mg | Campylobacter jejuni recombinant outer membrane protein |
MT-25CEPsl | 空肠弯曲杆菌重组外膜蛋白 | x100μg | Campylobacter jejuni recombinant outer membrane protein |
MT-25A1G | 贾第虫肠道滋养体重组蛋白 | x1mg | Giardia intestinalis trophozoite recombinant protein |
MT-25A1Gsl | 贾第虫肠道滋养体重组蛋白 | x100μg | Giardia intestinalis trophozoite recombinant protein |
MT-25GCP | 贾第虫肠囊菌重组蛋白 | x1mg | Giardia intestinalis cyst recombinant protein |
MT-25GCPsl | 贾第虫肠囊菌重组蛋白 | x100μg | Giardia intestinalis cyst recombinant protein |
MT-25EDP | 内阿米巴重组蛋白 | x1mg | Entamoeba dispar recombinant protein |
MT-25EDPsl | 内阿米巴重组蛋白 | x100μg | Entamoeba dispar recombinant protein |
MT-25EHP | 溶组织内阿米巴重组蛋白 | x1mg | Entamoeba histolytica recombinant protein |
MT-25EHPsl | 溶组织内阿米巴重组蛋白 | x100μg | Entamoeba histolytica recombinant protein |
MT-25HCP | 人类钙卫蛋白重组蛋白 | x1mg | Human Calprotectin recombinant protein |
MT-25HCPs1 | 人类钙卫蛋白重组蛋白 | x100μg | Human Calprotectin recombinant protein |
MT-31NGA | 诺如病毒GI.1重组VLP | x1mg | Norovirus GI.1 recombinant VLP |
MT-31NGAsl | 诺如病毒GI.1重组VLP | x100μg | Norovirus GI.1 recombinant VLP |
MT-31NPA | 诺如病毒GII.4重组VLP | x1mg | Norovirus GII.4 recombinant VLP |
MT-31NPAsl | 诺如病毒GII.4重组VLP | x100μg | Norovirus GII.4 recombinant VLP |
MT-28PECU | 灭活的幽门螺杆菌抗原(天然提取物) | x1mg | Inactivated H. pylori antigen (native extract) |
MT-28PECUsl | 灭活的幽门螺杆菌抗原(天然提取物) | x100μg | Inactivated H. pylori antigen (native extract) |
MT-28EC7U | 灭活的大肠杆菌O157抗原(天然提取物) | x1mg | Inactivated E. coli O157 antigen (native extract) |
MT-28EC7Usl | 灭活的大肠杆菌O157抗原(天然提取物) | x100μg | Inactivated E. coli O157 antigen (native extract) |
MT-28CCU | 灭活的大肠杆菌抗原(天然提取物) | x1mg | Inactivated Campylobacter coli antigen (native extract) |
MT-28CCUsl | 灭活的大肠杆菌抗原(天然提取物) | x100μg | Inactivated Campylobacter coli antigen (native extract) |
MT-28CJU | 灭活的空肠弯曲杆菌抗原(天然提取物) | x1mg | Inactivated Campylobacter jejuni antigen (native extract) |
MT-28CJUsl | 灭活的空肠弯曲杆菌抗原(天然提取物) | x100μg | Inactivated Campylobacter jejuni antigen (native extract) |
MT-28SEU | 灭活肠炎沙门氏菌抗原(天然提取物) | x1mg | Inactivated Salmonella enteritidis antigen (native extract) |
MT-28SEUs1 | 灭活肠炎沙门氏菌抗原(天然提取物) | x100μg | Inactivated Salmonella enteritidis antigen (native extract) |
MT-28SPAU | 灭活沙门氏菌副伤寒A抗原(天然提取物) | x1mg | Inactivated Salmonella paratyphi A antigen (native extract) |
MT-28SPAUsl | 灭活沙门氏菌副伤寒A抗原(天然提取物) | x100μg | Inactivated Salmonella paratyphi A antigen (native extract) |
MT-28SPBU | 灭活沙门氏菌副伤寒B抗原(天然提取物) | x1mg | Inactivated Salmonella paratyphi B antigen (native extract) |
MT-28SPBUsl | 灭活沙门氏菌副伤寒B抗原(天然提取物) | x100μg | Inactivated Salmonella paratyphi B antigen (native extract) |
MT-28STMU | 灭活的鼠伤寒沙门氏菌抗原(天然提取物) | x1mg | Inactivated Salmonella typhimurium antigen (native extract) |
MT-28STMUsl | 灭活的鼠伤寒沙门氏菌抗原(天然提取物) | x100μg | Inactivated Salmonella typhimurium antigen (native extract) |
MT-28STU | 灭活伤寒沙门氏菌抗原(天然提取物) | x1mg | Inactivated Salmonella typhi antigen (native extract) |
MT-28STUsl | 灭活伤寒沙门氏菌抗原(天然提取物) | x100μg | Inactivated Salmonella typhi antigen (native extract) |
MT-28LMU | 灭活的单核细胞增生李斯特菌抗原(天然提取物) | x1mg | Inactivated Listeria monocytogenes antigen (native extract) |
MT-28LMUsl | 灭活的单核细胞增生李斯特菌抗原(天然提取物) | x100μg | Inactivated Listeria monocytogenes antigen (native extract) |
MT-28YE3U | 灭活小肠结肠炎耶尔森氏菌O:3抗原(天然提取物) | x1mg | Inactivated Yersinia enterocolitica O:3 antigen (native extract) |
MT-28YE3Usl | 灭活小肠结肠炎耶尔森氏菌O:3抗原(天然提取物) | x100μg | Inactivated Yersinia enterocolitica O:3 antigen (native extract) |
MT-28YE9U | 灭活小肠结肠炎耶尔森氏菌O:9抗原(天然提取物) | x1mg | Inactivated Yersinia enterocolitica O:9 antigen (native extract) |
MT-28YE9Usl | 灭活小肠结肠炎耶尔森氏菌O:9抗原(天然提取物) | x100μg | Inactivated Yersinia enterocolitica O:9 antigen (native extract) |
MT-28SBU | 灭活的鲍氏志贺氏菌抗原(天然提取物) | x1mg | Inactivated Shigella boydii antigen (native extract) |
MT-28SBUsl | 灭活的鲍氏志贺氏菌抗原(天然提取物) | x100μg | Inactivated Shigella boydii antigen (native extract) |
MT-28SDU | 灭活的痢疾志贺氏菌抗原(天然提取物) | x1mg | Inactivated Shigella dysenteriae antigen (native extract) |
MT-28SDUsl | 灭活的痢疾志贺氏菌抗原(天然提取物) | x100μg | Inactivated Shigella dysenteriae antigen (native extract) |
MT-28SFU | 灭活的福氏志贺氏菌抗原(天然提取物) | x1mg | Inactivated Shigella flexneri antigen (native extract) |
MT-28SFUdl | 灭活的福氏志贺氏菌抗原(天然提取物) | x100μg | Inactivated Shigella flexneri antigen (native extract) |
MT-28SSU | 灭活的宋内氏志贺菌抗原(天然提取物) | x1mg | Inactivated Shigella sonnei antigen (native extract) |
MT-28SSUsl | 灭活的宋内氏志贺菌抗原(天然提取物) | x100μg | Inactivated Shigella sonnei antigen (native extract) |
MT-29KOE | 灭活小球隐孢子虫抗原(天然提取物) | x1mg | Inactivated Cryptosporidium parvum antigen (native extract) |
MT-29KOEsl | 灭活小球隐孢子虫抗原(天然提取物) | x100μg | Inactivated Cryptosporidium parvum antigen (native extract) |
MT-29HHB | 人血红蛋白蛋白质(天然提取物) | x1mg | Human Haemoglobin protein (native extract) |
MT-29HHBsl | 人血红蛋白蛋白质(天然提取物) | x100μg | Human Haemoglobin protein (native extract) |
MT-29HLF | 人乳铁蛋白蛋白质(天然提取物) | x1mg | Human Lactoferrin protein (native extract) |
MT-29HLF | 人乳铁蛋白蛋白质(天然提取物) | x100μg | Human Lactoferrin protein (native extract) |
MT-29HTF | 人转铁蛋白蛋白质(天然提取物) | x1mg | Human Transferrin protein (native extract) |
MT-29HTFsl | 人转铁蛋白蛋白质(天然提取物) | x100μg | Human Transferrin protein (native extract) |
MT-20TSS | 溶血性A链球菌抗体 | x1mg | Anti-Strep A Pab |
MT-20TSSsl | 溶血性A链球菌抗体 | x100μg | Anti-Strep A Pab |
MT-18RV3 | 呼吸道合胞病毒单抗(克隆RV3) | x1mg | Anti-RSV Mab (clone RV3) |
MT-18RV3sl | 呼吸道合胞病毒单抗(克隆RV3) | x100μg | Anti-RSV Mab (clone RV3) |
MT-18RV4 | 呼吸道合胞病毒单抗(克隆RV4) | x1mg | Anti-RSV Mab (clone RV4) |
MT-18RV4sl | 呼吸道合胞病毒单抗(克隆RV4) | x100μg | Anti-RSV Mab (clone RV4) |
MT-18A14 | 腺病毒单克隆抗体(克隆A14) | x1mg | Anti-Adenovirus Mab (clone A14) |
MT-18A14sl | 腺病毒单克隆抗体(克隆A14) | x100μg | Anti-Adenovirus Mab (clone A14) |
MT-18A15 | 腺病毒单克隆抗体(克隆A15) | x1mg | Anti-Adenovirus Mab (clone A15) |
MT-18A15Rsl | 腺病毒单克隆抗体(克隆A15) | x100μg | Anti-Adenovirus Mab (clone A15) |
MT-18Y77 | 甲型流感病毒单抗(克隆Y77) | x1mg | Anti-Influenza A Mab (clone Y77) |
MT-18Y77sl | 甲型流感病毒单抗(克隆Y77) | x100μg | Anti-Influenza A Mab (clone Y77) |
MT-18YB91 | 乙型流感病毒单抗(克隆YB91) | x1mg | Anti-Influenza B Mab (clone YB91) |
MT-18YB91sl | 乙型流感病毒单抗(克隆YB91) | x100μg | Anti-Influenza B Mab (clone YB91) |
MT-18LN14 | 嗜肺军团菌单抗(克隆LN14) | x1mg | Anti-Legionella pneumophila Mab (clone LN14) |
MT-18LN14sl | 嗜肺军团菌单抗(克隆LN14) | x100μg | Anti-Legionella pneumophila Mab (clone LN14) |
MT-18LN29 | 嗜肺军团菌单抗(克隆LN29) | x1mg | Anti-Legionella pneumophila Mab (clone LN29) |
MT-18LN29sl | 嗜肺军团菌单抗(克隆LN29) | x100μg | Anti-Legionella pneumophila Mab (clone LN29) |
MT-18SN3 | 肺炎链球菌单克隆抗体(克隆SN3) | x1mg | Anti-Streptococcus pneumoniae Mab (clone SN3) |
MT-18SN3s1 | 肺炎链球菌单克隆抗体(克隆SN3) | x100μg | Anti-Streptococcus pneumoniae Mab (clone SN3) |
MT-18SN4 | 肺炎链球菌单克隆抗体(克隆SN4) | x1mg | Anti-Streptococcus pneumoniae Mab (clone SN4) |
MT-18SN4sl | 肺炎链球菌单克隆抗体(克隆SN4) | x100μg | Anti-Streptococcus pneumoniae Mab (clone SN4) |
MT-25RSV | 呼吸道合胞病毒重组融合蛋白 | x1mg | RSV recombinant fusion protein |
MT-25RSVsl | 呼吸道合胞病毒重组融合蛋白 | x100μg | RSV recombinant fusion protein |
MT-25HEXR | 腺病毒六邻体重组蛋白 | x1mg | Adenovirus HEXON recombinant protein |
MT-25HEXRsl | 腺病毒六邻体重组蛋白 | x100μg | Adenovirus HEXON recombinant protein |
MT-25FAN | 甲型流感病毒重组核蛋白 | x1mg | Influenza A recombinant nucleoprotein |
MT-25FANsl | 甲型流感病毒重组核蛋白 | x100μg | Influenza A recombinant nucleoprotein |
MT-25FBN | 乙型流感病毒重组核蛋白 | x1mg | Influenza B recombinant nucleoprotein |
MT-25FBNsl | 乙型流感病毒重组核蛋白 | x100μg | Influenza B recombinant nucleoprotein |
MT-28SAGU | 灭活A链球菌抗原(天然提取物) | x1mg | Inactivated STREP A antigen (native extract) |
MT-28SAGUsl | 灭活A链球菌抗原(天然提取物) | x100μg | Inactivated STREP A antigen (native extract) |
MT-29RVV | 灭活呼吸道合胞病毒抗原(天然提取物) | x1mg | Inactivated RSV antigen (native extract) |
MT-29RVVsl | 灭活呼吸道合胞病毒抗原(天然提取物) | x100μg | Inactivated RSV antigen (native extract) |
MT-28LNU | 灭活的嗜肺军团菌抗原(天然提取物) | x1mg | Inactivated Legionella pneumophila antigen (native extract) |
MT-28LNUsl | 灭活的嗜肺军团菌抗原(天然提取物) | x100μg | Inactivated Legionella pneumophila antigen (native extract) |
MT-28SPNU | 灭活的肺炎链球菌抗原(天然提取物) | x1mg | Inactivated Streptococcus pneumoniae antigen (native extract) |
MT-28SPNUsl | 灭活的肺炎链球菌抗原(天然提取物) | x100μg | Inactivated Streptococcus pneumoniae antigen (native extract) |
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3、zui大简约法(Maximum parsimony,MP):
zui早源于形态性状研究,现在已推广到分子序列的进化分析中。zui大简约法的理论基础是奥卡姆(Ockham)哲学原则,这个原则认为:解释一个过程的理论是所需假设数目zui少的那一个。对所有可能的拓扑结构进行计算,并计算出所需替代数zui小的那个拓扑结构,作为*树。优点:zui大简约法对于分析某些特殊的分子数据,如插入、缺失等序列有用。在分析的序列位点上没有回复突变或平行突变,且被检验的序列位点数很大的时候,zui大简约法能够推导获得一个很好的进化树。缺点:在分析序列上存在较多的回复突变或平行突变,而被检验的序列位点数又比较少的时候,zui大简约法可能会给出一个不合理的或者错误的进化树推导结果。
一般来讲,如果模型合适,ML的效果较好。对近缘序列,有人喜欢MP,因为用的假设zui少。MP一般不用在远缘序列上,这时一般用NJ或ML。对相似度很低的序列,NJ往往出现Long-branch attraction(LBA,长枝吸引现象),有时严重干扰进化树的构建。贝叶斯的方法则太慢。对于各种方法构建分子进化树的准确性,一篇综述(Hall BG. Mol Biol Evol 2005, 22(3):792-802)认为贝叶斯的方法,其次是ML,然后是MP。其实如果序列的相似性较高,各种方法都会得到不错的结果,模型间的差别也不大。
对于NJ和ML,是需要选择模型的。对于蛋白质序列以及DNA序列,两者模型的选择是不同的。对于蛋白质的序列,一般选择Poisson Correction(泊松修正)这一模型。而对于核酸序列,一般选择Kimura 2-parameter(Kimura-2参数)模型。如果对各种模型的理解并不深入,不推荐初学者使用其他复杂的模型。
BOOTSTRAP值即自展值,可用来检验所计算的进化树分支可信度。Bootstrap几乎是构建系统进化树一个必须的选项。一般Bootstrap的值>70%,则认为构建的进化树较为可靠。如果Bootstrap的值太低,则有可能进化树的拓扑结构有错误,进化树是不可靠的。
Bootstrap值是指根据所选的统计计算模型,设定初始值1000次,就是把序列的位点都重排,重排后的序列再用相同的办法构树,如此让模型计算并绘制1000株系统发育树,这是命令阶段产生的。如果原来树的分枝在重排后构的树中也出现了,就给这个分枝打上1分,如果没出现就给0分,这样给进化树打分后,每个分枝就都得出分值。系统发育树中每个节点上的数字则代表在命令阶段要求的1000次进化树分析中,有多少次。重排的序列有很多组合,值越小说明分枝的可信度越低,根据数据的情况选用不同的构树方法和模型。比如鉴定菌种时一般认为节点数字初始设置1000计算后显示大于500(有的时候显示是百分数,要注意)时,这样的系统发育分析才具有可信度,学术杂志才会接收或者认可。
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3, the maximum parsimony (Maximum parsimony, MP):
Earliest from morphological traits, has now been extended to the evolutionary analysis of molecular sequences. The theoretical basis of Maximal Minimalist Law is the Ockham philosophy, which states that the best theory to explain a process is the one that requires the least number of hypotheses. Calculate all possible topologies and calculate the topology with the smallest number of substitutions as the optimal tree. Advantages: Maximum parsimony is useful for analyzing certain molecular data, such as insertions, deletions, etc. When there are no back mutations or parallel mutations in the analyzed sequence sites, and the number of sequence sites tested is large, the maximum parsimony method can be deduced to obtain a good phylogenetic tree. Disadvantages: When there are many recovery mutations or parallel mutations in the analysis sequence, and the number of sequences to be examined is relatively small, the maximum parsimony method may give an unreasonable or wrong evolutionary tree derivation result.
In general, ML works better if the model is fit. Some people like MPs for the near-miss sequences, because they use the least assumptions. MP is generally not used in the distant sequence, then generally use NJ or ML. For sequences with low similarity, NJ often has Long-branch attraction (LBA), which sometimes seriously interferes with the construction of phylogenetic trees. Bayesian approach is too slow. A review (Hall BG. Mol Biol Evol 2005, 22 (3): 792-802) considers Bayes' approach to be the best, followed by ML and then MP, for the accuracy of the various approaches for constructing molecular phylogenetic trees. In fact, if the similarity of sequences is high, all kinds of methods will get good results, and the difference between the models is not big.
For NJ and ML, it is necessary to choose the model. For protein sequences and DNA sequences, the choice between the two models is different. For protein sequences, the Poisson Correction model is generally chosen. For nucleic acid sequences, the Kimura 2-parameter model is generally chosen. If the understanding of various models is not deep, it is not recommended for beginners to use other complex models.
The BOOTSTRAP value is the self-scaling value that can be used to test the confidence of the calculated branch of the tree. Bootstrap is almost a necessary option for building a phylogenetic tree. General Bootstrap value> 70%, then the construction of the evolutionary tree that more reliable. If the value of Bootstrap is too low, then there is a possibility that the topology of the evolutionary tree is wrong and the evolutionary tree is not reliable.
Bootstrap value is based on the selected statistical model, set the initial value of 1000 times, is to rearrange the sequence of sites, the rearranged sequence and then use the same way to tree, so that the model to calculate and draw 1000 Phylogenetic tree, which is produced during the command phase. If the original tree branches in the rearranged structure of the tree also appeared, give this branch labeled 1 point, if not give 0 points, so after the evolutionary tree scoring, each branch have come Score. The number at each node in the phylogenetic tree represents how many times in the 1000 phylogenetic analyzes required in the command phase. There are many combinations of rearranged sequences, the smaller the value indicates the lower the credibility of the branch, the best according to the situation of the data to choose different tree methods and models. Such as the identification of species is generally believed that the initial calculation of the number of nodes displayed after the calculation of more than 500 (sometimes shown as a percentage, pay attention), such a phylogenetic analysis only has the credibility of academic journals will receive or endorse.