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西班牙Certest腺病毒六邻体重组蛋白
广州健仑生物科技有限公司
广州健仑长期供应各种生物原料，主要代理品牌：西班牙Certest。
Certest Biotec，S.L. 是一家独立的生物技术公司，致力于人类临床领域的IVD诊断产品的开发和制造。快速检测是基于快速、准确和易于操作的诊断产品。 此外，Cerstest还有多种实时PCR产品，可以用于医院临床、实验室科研等。
Certest公司于2002年在萨拉戈萨成立，是一家创新技术型公司。公司的发展是基于新产品的开发、市场空间和机遇的探索，十几年来一直以高度专业化，以客户为导向，不断得创新，优化产品，专业知识，取得广大客户的信任和支持。Certest的质量体系已通过ISO 13485认证。
主要产品包括各种生物单克隆抗原抗体、重组蛋白。
轮状病毒单克隆抗体、腺病毒抗体、星状病毒单克隆抗体、诺如病毒单克隆抗体、幽门螺旋杆菌抗体、隐球菌抗体、肠道病毒抗体、贾第鞭毛虫抗体、弯曲杆菌抗体、阿米巴原虫抗体、呼吸道合胞病毒单抗等等。
西班牙Certest腺病毒六邻体重组蛋白
我司还提供其它进口或国产试剂盒：登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。
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西班牙Certest公司简介
Certest Biotec, S.L. is an independent biotechnology company devoted to the development and manufacturing of IVD diagnostic products in human clinical field. Our Rapid Test are based in a fast, reliable and easy-interpretation process. In addition, VIASURE contains a wide range of products for Real Time PCR that allow to identify the causal pathogens of the infectious diseases in physicians’ offices, labs and hospitals.
The company was established in Zaragoza in 2002 as an innovative and technology-based company. The company’s growth is based on the development of new products and the exploration for new market niches and opportunities. The key to understand its success is the way to understand its organization: highly specialised and customer-oriented based.
Mission, Vision, Values and Human Team
Mission
To develop, produce and market a wide range of innovative solutions and quality in vitro diagnostics for the detection of viruses, bacteria and parasites.
Vision
Being the international leader company in research and development for in vitro diagnostic solutions, increasing our distribution network through added value creation and company-customer trust.
Values
Customer orientation · Ethical behaviour · Teamwork · Continuous research and innovation ·Trained and motivated team · Quality and creativity in all processes
Human team
The multi-skilled team is a dedicated and experience professional group responsible for dealing with all the different company activities in an international environment.
Commitment to Innovation, Export and High-Technology.
Certest’s Quality System has been certified with ISO 13485.
One of the main objectives of Certest is to achieve customer satisfaction. For this, the management of the company ensures that customer requirements are determined and reviewed, identifying their needs and expectations, and translating them into requirements, all in order to maintain customer satisfaction.
See/Download the Certest Biotec ISO Standard Certification Certest Policy is consistent with its primary purpose, that of getting to all its products and services, quality and reliability, meeting the requirements and maintaining the effectiveness of the management system.
“Comparative study of two diagnostic tests for detection of RNA Zika Virus (ZIKV) in clinical samples”
“Comparison of Real-Time PCR test with the routine diagnosis technique to detect enteric pathogenic protozoa”
“Diagnostic of the Enteric Virus infection by Real-Time PCR in stool samples”
“Clinical performance evaluation of lyophilized VIASURE Real Time PCR Detection Kits for pathogen detection by participation in External Quality Assessment (EQA) programs”
“Detection and differentiation of Influenza A Virus, Influenza B Virus and H1N1 (pdm09) variant through an stabilized Real Time PCR Mix”
“Comparison of different molecular methods for the detection of non-influenza respiratory virus”virus”
【西班牙Certest生物原料】
货号 | 产品名称 | 规格 | 英文名称 |
MT-16R15 | 轮状病毒单克隆抗体（克隆R15） | x1mg | Anti-Rotavirus Mab (clone R15) |
MT-16R15s1 | 轮状病毒单克隆抗体（克隆R15） | x100μg | Anti-Rotavirus Mab (clone R15) |
MT-18A15 | 腺病毒抗体（克隆A15） | x1mg | Anti-Adenovirus Mab (clone A15) |
MT-18A15s1 | 腺病毒抗体（克隆A15） | x100μg | Anti-Adenovirus Mab (clone A15) |
MT-18AT18 | 星状病毒单克隆抗体（克隆AT18） | x1mg | Anti-Astrovirus Mab (clone AT18) |
MT-18AT18s1 | 星状病毒单克隆抗体（克隆AT18） | x100μg | Anti-Astrovirus Mab (clone AT18) |
MT-18AT8 | 星状病毒单克隆抗体（克隆AT8） | x1mg | Anti-Astrovirus Mab (clone AT8) |
MT-18AT8s1 | 星状病毒单克隆抗体（克隆AT8） | x100μg | Anti-Astrovirus Mab (clone AT8) |
MT-18NG28 | 诺如病毒GI单克隆抗体（克隆NG28） | x1mg | Anti-Norovirus GI Mab (clone NG28) |
MT-18NG28sl | 诺如病毒GI单克隆抗体（克隆NG28） | x100μg | Anti-Norovirus GI Mab (clone NG28) |
MT-18NP8 | 诺如病毒GII单克隆抗体（克隆NP8） | x1mg | Anti-Norovirus GII Mab (clone NP8) |
MT-18NP8sl | 诺如病毒GII单克隆抗体（克隆NP8） | x100μg | Anti-Norovirus GII Mab (clone NP8) |
MT-18EV5 | 肠道病毒抗体（克隆EV5） | x1mg | Anti-Enterovirus Mab (clone EV5) |
MT-18EV5sl | 肠道病毒抗体（克隆EV5） | x100μg | Anti-Enterovirus Mab (clone EV5) |
MT-16P2 | 幽门螺旋杆菌抗体（克隆P2）HP抗体 | x1mg | Anti-H. pylori Mab (clone P2) |
MT-16P2sl | 幽门螺旋杆菌抗体（克隆P2）HP抗体 | x100μg | Anti-H. pylori Mab (clone P2) |
MT-16GD10 | 艰难梭菌抗体（克隆GD10） | x1mg | Anti-GDH Mab (clone GD10) |
MT-16GD10sl | 艰难梭菌抗体（克隆GD10） | x100μg | Anti-GDH Mab (clone GD10) |
MT-18TA5 | 艰难梭菌毒素A抗（克隆TA5） | x1mg | Anti-CD Toxin A Mab (clone TA5) |
MT-18TA5sl | 艰难梭菌毒素A抗（克隆TA5） | x100μg | Anti-CD Toxin A Mab (clone TA5) |
MT-18TA7 | 艰难梭菌毒素A抗（克隆TA7） | x1mg | Anti-CD Toxin A Mab (clone TA7) |
MT-18TA7sl | 艰难梭菌毒素A抗（克隆TA7） | x100μg | Anti-CD Toxin A Mab (clone TA7) |
MT-18TB41 | 艰难梭菌毒素B抗（克隆TB41） | x1mg | Anti-CD Toxin B Mab (clone TB41) |
MT-18TB41sl | 艰难梭菌毒素B抗（克隆TB41） | x100μg | Anti-CD Toxin B Mab (clone TB41) |
MT-18TB48 | 艰难梭菌毒素B抗（克隆TB48） | x1mg | Anti-CD Toxin B Mab (clone TB48) |
MT-18TB48sl | 艰难梭菌毒素B抗（克隆TB48） | x100μg | Anti-CD Toxin B Mab (clone TB48) |
MT-18E10 | 大肠杆菌O157抗体（克隆E10） | x1mg | Anti-E. coli O157 Mab (clone E10) |
MT-18E10sl | 大肠杆菌O157抗体（克隆E10） | x100μg | Anti-E. coli O157 Mab (clone E10) |
MT-16CA29 | 弯曲杆菌抗体（克隆ECA29） | x1mg | Anti-Campylobacter Mab (clone CA29) |
MT-16CA29sl | 弯曲杆菌抗体（克隆ECA29） | x100μg | Anti-Campylobacter Mab (clone CA29) |
MT-18K31 | 隐球菌抗体（克隆K31） | x1mg | Anti-Crypto Mab (clone K31) |
MT-18K31sl | 隐球菌抗体（克隆K31） | x100μg | Anti-Crypto Mab (clone K31) |
MT-16G18 | 贾第鞭毛虫抗体（克隆G18） | x1mg | Anti-Giardia Mab trophozoite protein (clone G18) |
MT-16G18sl | 贾第鞭毛虫抗体（克隆G18） | x100μg | Anti-Giardia Mab trophozoite protein (clone G18) |
MT-16G22 | 贾第鞭毛虫抗体（克隆G22） | x1mg | Anti-Giardia Mab trophozoite protein (clone G22) |
MT-16G22sl | 贾第鞭毛虫抗体（克隆G22） | x100μg | Anti-Giardia Mab trophozoite protein (clone G22) |
MT-18EH30 | 阿米巴原虫抗体（克隆H30） | x1mg | Anti-Entamoeba Mab (clone EH30) |
MT-18EH30sl | 阿米巴原虫抗体（克隆H30） | x100μg | Anti-Entamoeba Mab (clone EH30) |
MT-16CP14 | 钙结合蛋白单克隆抗体（克隆CP14） | x1mg | Anti-Calprotectin Mab (clone CP14) |
MT-16CP14sl | 钙结合蛋白单克隆抗体（克隆CP14） | x100μg | Anti-Calprotectin Mab (clone CP14) |
MT-16F22 | 血红蛋白单抗（克隆F22） | x1mg | Anti-Haemoglobin Mab (clone F22) |
MT-16F22sl | 血红蛋白单抗（克隆F22） | x100μg | Anti-Haemoglobin Mab (clone F22) |
MT-16LC16 | 乳铁蛋白单抗（克隆LC16） | x1mg | Anti-Lactoferrin Mab (clone LC16) |
MT-16LC16sl | 乳铁蛋白单抗（克隆LC16） | x100μg | Anti-Lactoferrin Mab (clone LC16) |
MT-16LC4 | 乳铁蛋白单抗（克隆LC4） | x1mg | Anti-Lactoferrin Mab (clone LC4) |
MT-16LC4sl | 乳铁蛋白单抗（克隆LC4） | x100μg | Anti-Lactoferrin Mab (clone LC4) |
MT-26VP6 | 轮状病毒VP6重组蛋白 | x1mg | Rotavirus VP6 recombinant protein |
MT-26VP6 | 轮状病毒VP6重组蛋白 | x100μg | Rotavirus VP6 recombinant protein |
MT-25HEX | 腺病毒HEXON重组蛋白 | x1mg | Adenovirus HEXON recombinant protein |
MT-25HEXsl | 腺病毒HEXON重组蛋白 | x100μg | Adenovirus HEXON recombinant protein |
MT-25AST | 星状病毒衣壳重组蛋白 | x1mg | Astrovirus capsid recombinant protein |
MT-25ASTsl | 星状病毒衣壳重组蛋白 | x100μg | Astrovirus capsid recombinant protein |
MT-25NGI1 | 诺如病毒GI.1重组P结构域 | x1mg | Norovirus GI.1 recombinant P domain |
MT-25NGI1sl | 诺如病毒GI.1重组P结构域 | x100μg | Norovirus GI.1 recombinant P domain |
MT-25NGI3 | 诺如病毒GI.3重组P结构域 | x1mg | Norovirus GI.3 recombinant P domain |
MT-25NGI3sl | 诺如病毒GI.3重组P结构域 | x100μg | Norovirus GI.3 recombinant P domain |
MT-25NGII10 | 诺如病毒GII.10重组P结构域 | x1mg | Norovirus GII.10 recombinant P domain |
MT-25NGII10sl | 诺如病毒GII.10重组P结构域 | x100μg | Norovirus GII.10 recombinant P domain |
MT-25NGII17 | 诺如病毒GII.17重组P结构域 | x1mg | Norovirus GII.17 recombinant P domain |
MT-25NGII17sl | 诺如病毒GII.17重组P结构域 | x100μg | Norovirus GII.17 recombinant P domain |
MT-25NGII14 | 诺如病毒GII.4重组P结构域 | x1mg | Norovirus GII.4 recombinant P domain |
MT-25NGII14sl | 诺如病毒GII.4重组P结构域 | x100μg | Norovirus GII.4 recombinant P domain |
MT-25ETV | 肠道病毒VP1重组蛋白 | x1mg | Enterovirus VP1 recombinant protein |
MT-25ETVs1 | 肠道病毒VP1重组蛋白 | x100μg | Enterovirus VP1 recombinant protein |
MT-25PCH | 幽门螺杆菌重组外膜蛋白 | x1mg | H. pylori recombinant outer membrane protein |
MT-25PCHs1 | 幽门螺杆菌重组外膜蛋白 | x100μg | H. pylori recombinant outer membrane protein |
MT-25GDH | 艰难梭菌GDH重组蛋白 | x1mg | Clostridium difficile GDH recombinant protein |
MT-25GDHsl | 艰难梭菌GDH重组蛋白 | x100μg | Clostridium difficile GDH recombinant protein |
MT-24TXA | 艰难梭菌毒素A重组蛋白（无毒性片段） | x1mg | C. difficile Toxin A recombinant protein (fragment without toxic activity) |
MT-24TXAsl | 艰难梭菌毒素A重组蛋白（无毒性片段） | x100μg | C. difficile Toxin A recombinant protein (fragment without toxic activity) |
MT-24TXB | 艰难梭菌毒素B重组蛋白（无毒性片段） | x1mg | C. difficile Toxin B recombinant protein (fragment without toxic activity) |
MT-24TXBsl | 艰难梭菌毒素B重组蛋白（无毒性片段） | x100μg | C. difficile Toxin B recombinant protein (fragment without toxic activity) |
MT-25STX | 大肠杆菌O157 VT1重组蛋白 | x1mg | E. coli O157 VT1 recombinant protein |
MT-25STXsl | 大肠杆菌O157 VT1重组蛋白 | x100μg | E. coli O157 VT1 recombinant protein |
MT-25VT2 | 大肠杆菌O157 VT2重组蛋白 | x1mg | E. coli O157 VT2 recombinant protein |
MT-25VT2sl | 大肠杆菌O157 VT2重组蛋白 | x100μg | E. coli O157 VT2 recombinant protein |
MT-25CCP | 弯曲杆菌重组外膜蛋白 | x1mg | Campylobacter coli recombinant outer membrane protein |
MT-25CCPsl | 弯曲杆菌重组外膜蛋白 | x100μg | Campylobacter coli recombinant outer membrane protein |
MT-25CEP | 空肠弯曲杆菌重组外膜蛋白 | x1mg | Campylobacter jejuni recombinant outer membrane protein |
MT-25CEPsl | 空肠弯曲杆菌重组外膜蛋白 | x100μg | Campylobacter jejuni recombinant outer membrane protein |
MT-25A1G | 贾第虫肠道滋养体重组蛋白 | x1mg | Giardia intestinalis trophozoite recombinant protein |
MT-25A1Gsl | 贾第虫肠道滋养体重组蛋白 | x100μg | Giardia intestinalis trophozoite recombinant protein |
MT-25GCP | 贾第虫肠囊菌重组蛋白 | x1mg | Giardia intestinalis cyst recombinant protein |
MT-25GCPsl | 贾第虫肠囊菌重组蛋白 | x100μg | Giardia intestinalis cyst recombinant protein |
MT-25EDP | 内阿米巴重组蛋白 | x1mg | Entamoeba dispar recombinant protein |
MT-25EDPsl | 内阿米巴重组蛋白 | x100μg | Entamoeba dispar recombinant protein |
MT-25EHP | 溶组织内阿米巴重组蛋白 | x1mg | Entamoeba histolytica recombinant protein |
MT-25EHPsl | 溶组织内阿米巴重组蛋白 | x100μg | Entamoeba histolytica recombinant protein |
MT-25HCP | 人类钙卫蛋白重组蛋白 | x1mg | Human Calprotectin recombinant protein |
MT-25HCPs1 | 人类钙卫蛋白重组蛋白 | x100μg | Human Calprotectin recombinant protein |
MT-31NGA | 诺如病毒GI.1重组VLP | x1mg | Norovirus GI.1 recombinant VLP |
MT-31NGAsl | 诺如病毒GI.1重组VLP | x100μg | Norovirus GI.1 recombinant VLP |
MT-31NPA | 诺如病毒GII.4重组VLP | x1mg | Norovirus GII.4 recombinant VLP |
MT-31NPAsl | 诺如病毒GII.4重组VLP | x100μg | Norovirus GII.4 recombinant VLP |
MT-28PECU | 灭活的幽门螺杆菌抗原（天然提取物） | x1mg | Inactivated H. pylori antigen (native extract) |
MT-28PECUsl | 灭活的幽门螺杆菌抗原（天然提取物） | x100μg | Inactivated H. pylori antigen (native extract) |
MT-28EC7U | 灭活的大肠杆菌O157抗原（天然提取物） | x1mg | Inactivated E. coli O157 antigen (native extract) |
MT-28EC7Usl | 灭活的大肠杆菌O157抗原（天然提取物） | x100μg | Inactivated E. coli O157 antigen (native extract) |
MT-28CCU | 灭活的大肠杆菌抗原（天然提取物） | x1mg | Inactivated Campylobacter coli antigen (native extract) |
MT-28CCUsl | 灭活的大肠杆菌抗原（天然提取物） | x100μg | Inactivated Campylobacter coli antigen (native extract) |
MT-28CJU | 灭活的空肠弯曲杆菌抗原（天然提取物） | x1mg | Inactivated Campylobacter jejuni antigen (native extract) |
MT-28CJUsl | 灭活的空肠弯曲杆菌抗原（天然提取物） | x100μg | Inactivated Campylobacter jejuni antigen (native extract) |
MT-28SEU | 灭活肠炎沙门氏菌抗原（天然提取物） | x1mg | Inactivated Salmonella enteritidis antigen (native extract) |
MT-28SEUs1 | 灭活肠炎沙门氏菌抗原（天然提取物） | x100μg | Inactivated Salmonella enteritidis antigen (native extract) |
MT-28SPAU | 灭活沙门氏菌副伤寒A抗原（天然提取物） | x1mg | Inactivated Salmonella paratyphi A antigen (native extract) |
MT-28SPAUsl | 灭活沙门氏菌副伤寒A抗原（天然提取物） | x100μg | Inactivated Salmonella paratyphi A antigen (native extract) |
MT-28SPBU | 灭活沙门氏菌副伤寒B抗原（天然提取物） | x1mg | Inactivated Salmonella paratyphi B antigen (native extract) |
MT-28SPBUsl | 灭活沙门氏菌副伤寒B抗原（天然提取物） | x100μg | Inactivated Salmonella paratyphi B antigen (native extract) |
MT-28STMU | 灭活的鼠伤寒沙门氏菌抗原（天然提取物） | x1mg | Inactivated Salmonella typhimurium antigen (native extract) |
MT-28STMUsl | 灭活的鼠伤寒沙门氏菌抗原（天然提取物） | x100μg | Inactivated Salmonella typhimurium antigen (native extract) |
MT-28STU | 灭活伤寒沙门氏菌抗原（天然提取物） | x1mg | Inactivated Salmonella typhi antigen (native extract) |
MT-28STUsl | 灭活伤寒沙门氏菌抗原（天然提取物） | x100μg | Inactivated Salmonella typhi antigen (native extract) |
MT-28LMU | 灭活的单核细胞增生李斯特菌抗原（天然提取物） | x1mg | Inactivated Listeria monocytogenes antigen (native extract) |
MT-28LMUsl | 灭活的单核细胞增生李斯特菌抗原（天然提取物） | x100μg | Inactivated Listeria monocytogenes antigen (native extract) |
MT-28YE3U | 灭活小肠结肠炎耶尔森氏菌O:3抗原（天然提取物） | x1mg | Inactivated Yersinia enterocolitica O:3 antigen (native extract) |
MT-28YE3Usl | 灭活小肠结肠炎耶尔森氏菌O:3抗原（天然提取物） | x100μg | Inactivated Yersinia enterocolitica O:3 antigen (native extract) |
MT-28YE9U | 灭活小肠结肠炎耶尔森氏菌O:9抗原（天然提取物） | x1mg | Inactivated Yersinia enterocolitica O:9 antigen (native extract) |
MT-28YE9Usl | 灭活小肠结肠炎耶尔森氏菌O:9抗原（天然提取物） | x100μg | Inactivated Yersinia enterocolitica O:9 antigen (native extract) |
MT-28SBU | 灭活的鲍氏志贺氏菌抗原（天然提取物） | x1mg | Inactivated Shigella boydii antigen (native extract) |
MT-28SBUsl | 灭活的鲍氏志贺氏菌抗原（天然提取物） | x100μg | Inactivated Shigella boydii antigen (native extract) |
MT-28SDU | 灭活的痢疾志贺氏菌抗原（天然提取物） | x1mg | Inactivated Shigella dysenteriae antigen (native extract) |
MT-28SDUsl | 灭活的痢疾志贺氏菌抗原（天然提取物） | x100μg | Inactivated Shigella dysenteriae antigen (native extract) |
MT-28SFU | 灭活的福氏志贺氏菌抗原（天然提取物） | x1mg | Inactivated Shigella flexneri antigen (native extract) |
MT-28SFUdl | 灭活的福氏志贺氏菌抗原（天然提取物） | x100μg | Inactivated Shigella flexneri antigen (native extract) |
MT-28SSU | 灭活的宋内氏志贺菌抗原（天然提取物） | x1mg | Inactivated Shigella sonnei antigen (native extract) |
MT-28SSUsl | 灭活的宋内氏志贺菌抗原（天然提取物） | x100μg | Inactivated Shigella sonnei antigen (native extract) |
MT-29KOE | 灭活小球隐孢子虫抗原（天然提取物） | x1mg | Inactivated Cryptosporidium parvum antigen (native extract) |
MT-29KOEsl | 灭活小球隐孢子虫抗原（天然提取物） | x100μg | Inactivated Cryptosporidium parvum antigen (native extract) |
MT-29HHB | 人血红蛋白蛋白质（天然提取物） | x1mg | Human Haemoglobin protein (native extract) |
MT-29HHBsl | 人血红蛋白蛋白质（天然提取物） | x100μg | Human Haemoglobin protein (native extract) |
MT-29HLF | 人乳铁蛋白蛋白质（天然提取物） | x1mg | Human Lactoferrin protein (native extract) |
MT-29HLF | 人乳铁蛋白蛋白质（天然提取物） | x100μg | Human Lactoferrin protein (native extract) |
MT-29HTF | 人转铁蛋白蛋白质（天然提取物） | x1mg | Human Transferrin protein (native extract) |
MT-29HTFsl | 人转铁蛋白蛋白质（天然提取物） | x100μg | Human Transferrin protein (native extract) |
MT-20TSS | 溶血性A链球菌抗体 | x1mg | Anti-Strep A Pab |
MT-20TSSsl | 溶血性A链球菌抗体 | x100μg | Anti-Strep A Pab |
MT-18RV3 | 呼吸道合胞病毒单抗（克隆RV3） | x1mg | Anti-RSV Mab (clone RV3) |
MT-18RV3sl | 呼吸道合胞病毒单抗（克隆RV3） | x100μg | Anti-RSV Mab (clone RV3) |
MT-18RV4 | 呼吸道合胞病毒单抗（克隆RV4） | x1mg | Anti-RSV Mab (clone RV4) |
MT-18RV4sl | 呼吸道合胞病毒单抗（克隆RV4） | x100μg | Anti-RSV Mab (clone RV4) |
MT-18A14 | 腺病毒单克隆抗体（克隆A14） | x1mg | Anti-Adenovirus Mab (clone A14) |
MT-18A14sl | 腺病毒单克隆抗体（克隆A14） | x100μg | Anti-Adenovirus Mab (clone A14) |
MT-18A15 | 腺病毒单克隆抗体（克隆A15） | x1mg | Anti-Adenovirus Mab (clone A15) |
MT-18A15Rsl | 腺病毒单克隆抗体（克隆A15） | x100μg | Anti-Adenovirus Mab (clone A15) |
MT-18Y77 | 甲型流感病毒单抗（克隆Y77） | x1mg | Anti-Influenza A Mab (clone Y77) |
MT-18Y77sl | 甲型流感病毒单抗（克隆Y77） | x100μg | Anti-Influenza A Mab (clone Y77) |
MT-18YB91 | 乙型流感病毒单抗（克隆YB91） | x1mg | Anti-Influenza B Mab (clone YB91) |
MT-18YB91sl | 乙型流感病毒单抗（克隆YB91） | x100μg | Anti-Influenza B Mab (clone YB91) |
MT-18LN14 | 嗜肺军团菌单抗(克隆LN14) | x1mg | Anti-Legionella pneumophila Mab (clone LN14) |
MT-18LN14sl | 嗜肺军团菌单抗(克隆LN14) | x100μg | Anti-Legionella pneumophila Mab (clone LN14) |
MT-18LN29 | 嗜肺军团菌单抗(克隆LN29) | x1mg | Anti-Legionella pneumophila Mab (clone LN29) |
MT-18LN29sl | 嗜肺军团菌单抗(克隆LN29) | x100μg | Anti-Legionella pneumophila Mab (clone LN29) |
MT-18SN3 | 肺炎链球菌单克隆抗体(克隆SN3) | x1mg | Anti-Streptococcus pneumoniae Mab (clone SN3) |
MT-18SN3s1 | 肺炎链球菌单克隆抗体(克隆SN3) | x100μg | Anti-Streptococcus pneumoniae Mab (clone SN3) |
MT-18SN4 | 肺炎链球菌单克隆抗体(克隆SN4) | x1mg | Anti-Streptococcus pneumoniae Mab (clone SN4) |
MT-18SN4sl | 肺炎链球菌单克隆抗体(克隆SN4) | x100μg | Anti-Streptococcus pneumoniae Mab (clone SN4) |
MT-25RSV | 呼吸道合胞病毒重组融合蛋白 | x1mg | RSV recombinant fusion protein |
MT-25RSVsl | 呼吸道合胞病毒重组融合蛋白 | x100μg | RSV recombinant fusion protein |
MT-25HEXR | 腺病毒六邻体重组蛋白 | x1mg | Adenovirus HEXON recombinant protein |
MT-25HEXRsl | 腺病毒六邻体重组蛋白 | x100μg | Adenovirus HEXON recombinant protein |
MT-25FAN | 甲型流感病毒重组核蛋白 | x1mg | Influenza A recombinant nucleoprotein |
MT-25FANsl | 甲型流感病毒重组核蛋白 | x100μg | Influenza A recombinant nucleoprotein |
MT-25FBN | 乙型流感病毒重组核蛋白 | x1mg | Influenza B recombinant nucleoprotein |
MT-25FBNsl | 乙型流感病毒重组核蛋白 | x100μg | Influenza B recombinant nucleoprotein |
MT-28SAGU | 灭活A链球菌抗原（天然提取物） | x1mg | Inactivated STREP A antigen (native extract) |
MT-28SAGUsl | 灭活A链球菌抗原（天然提取物） | x100μg | Inactivated STREP A antigen (native extract) |
MT-29RVV | 灭活呼吸道合胞病毒抗原（天然提取物） | x1mg | Inactivated RSV antigen (native extract) |
MT-29RVVsl | 灭活呼吸道合胞病毒抗原（天然提取物） | x100μg | Inactivated RSV antigen (native extract) |
MT-28LNU | 灭活的嗜肺军团菌抗原（天然提取物） | x1mg | Inactivated Legionella pneumophila antigen (native extract) |
MT-28LNUsl | 灭活的嗜肺军团菌抗原（天然提取物） | x100μg | Inactivated Legionella pneumophila antigen (native extract) |
MT-28SPNU | 灭活的肺炎链球菌抗原（天然提取物） | x1mg | Inactivated Streptococcus pneumoniae antigen (native extract) |
MT-28SPNUsl | 灭活的肺炎链球菌抗原（天然提取物） | x100μg | Inactivated Streptococcus pneumoniae antigen (native extract) |
西班牙Certest腺病毒六邻体重组蛋白
提高测序覆盖率在一定程度上可以有效减少基因组中的Gap，但成本相对较高，并且对于一些复杂的微生物基因组效果有限，如454二代测序覆盖率为10×时，喜温硫杆菌SM-1测序后生成的Gap数目为400个；测序覆盖率提高至25×时，Gap数目减少至280个；但当测序覆盖率继续提高至38×时，Gap数目进入平台期(276个)，相比25×测序覆盖率时仅减少了4个，已经不能再单纯通过提高覆盖率来减少Gap数目。因此，对于复杂的微生物基因组，需要将基因组的Gap closure分为几个阶段，针对不同阶段采用相应的策略进行：如果Gap数目大于200个，可以通过构建基因组文库、Paired-End测序或者采用基因组光学图谱技术的策略确定Contig之间的相对位置和顺序，然后再依次关闭Contig之间的Gap区域；当Ga数目小于100个时，可以采用多引物PCR的策略寻找Linkage信息，关闭所有能够关闭的Gap；如果zui后还剩余几个Gap无法关闭，则可以采用基因组步移或文库筛选的策略，如在对喜温硫杆菌SM-1基因组Gap closure时，通过结合构建基因组文库、Paired-End测序、多引物PCR以及Fosimid文库筛选等多种策略zui终完成了SM-1基因组的Gap closure。
由于新一代测序技术测序读长较短、基因组结构的复杂性以及测序过程中的偏向性等原因，使得已完成测序的一些物种的基因组中含有数目不等的空缺区域。据统计，自2008年以来，GenBank释放的5276个微生物基因组序列中仅有32% (1692)是完整序列。基因组空缺区域中可能存在重要的生物学信息，如果不能补齐所有的Gap，不仅无法获得完整的基因组图谱，还会给后续的基因组信息解读(操纵子结构、基因调控、SNP分析以及比较基因组等)造成困难。因此，完整微生物基因组序列的获得需要在完成测序之后对空缺区域进行填充，即将测序拼装后生成的叠联群(Contig)之间的Gap进行填充，然后按照一定的次序和方向拼装生成一条完整的基因组序列(完成图)，这个过程称之为基因组的Gap closure (或补洞)。
基因组光学图谱技术是一种利用单分子DNA的限制性内切酶图谱生成高分辨率、有序的全基因组限制性内切酶酶切图谱的方法。
2010年，OpGen公司推出了基于光学图谱技术的自动化的基因组拼接和数据分析系统——Argus?工作站，能够在一个工作日内获得待测微生物基因组的高质量全基因组限制性内切酶酶切图谱。该系统主要由Mapcard加工工作站、光学扫描系统和数据处理工作站等组成，通过限制性内切酶对固定于MapCard DNA surface区域中的单分子DNA进行原位切割，使切割后的DNA片段顺序保持不变。DNA片段经荧光染料染色后置于荧光显微镜下，采集每个限制性内切酶片段的大小和顺序的信息，信息经转换处理后生成单个DNA分子的限制性内切酶酶切位点图谱，zui后根据全部DNA分子限制性内切酶酶切位点图谱的相互重叠部分拼接得到全基因组限制性内切酶酶切位点图谱。该系统zui初被应用于微生物基因组序列组装、比较基因组学以及菌株分类，目前已经开始在人类和动植物全基因组组装中应用。
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【公司名称】 广州健仑生物科技有限公司
【市场部】 杨永汉
【】
【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室
Increasing sequencing coverage can effectively reduce Gap in the genome to a certain extent, but the cost is relatively high, and the effect is limited for some complicated microbial genomes. For example, when the 454 second-generation sequencing coverage is 10 ×, 1, the number of Gap generated after sequencing was 400. When the coverage increased to 25 ×, the number of Gap decreased to 280. However, when the coverage of sequencing continued to increase to 38 ×, the number of Gap entered platform (276) Only a 4-fold decrease compared to 25 × sequencing coverage. It is no longer possible to reduce the number of Gaps simply by increasing the coverage. Therefore, for complex microbial genomes, it is necessary to divide the genomic Gap closure into several stages and adopt corresponding strategies for different stages. If the Gap number is more than 200, the Gap closure may be performed by constructing a genomic library, Paired-End sequencing, or using genomic optics The strategy of the map technology determines the relative position and order of the Contigs, and then turns off the Gap regions between the Contigs in sequence. When the number of Ga is less than 100, the strategy of the multi-primer PCR can be used to find the Linkage information and close all the Gaps ; If the last remaining few Gap can not be shut down, you can use the strategy of genome walking or library screening, such as in the Gap closure of the SM-1 genome of Thermus thermophilus by combining the construction of the genomic library, Paired-End sequencing, and more Primer PCR and Fosimid library screening strategies such as the final completion of the SM-1 genome Gap closure.
Due to the short sequencing length of new-generation sequencing technologies, the complexity of the genome structure and the bias in the sequencing process, some of the previously sequenced genomes contain a variable number of vacant genomes. According to statistics, only 32% (1692) of 5276 microbial genome sequences released by GenBank since 2008 are the complete sequences. There may be important biological information in the genomic locus where not all of the Gap can be populated, not only failing to obtain a complete genome map, but also interpreting subsequent genomic information (operon structure, gene regulation, SNP analysis, and comparative genomics Cause difficulties. Therefore, the complete microbial genome sequence needs to be filled after the completion of the sequencing of the vacancy region, that is to say, the Gap between contigs generated after sequencing and assembly is filled in, and then assembled according to a certain order and direction to generate a complete Genome sequence (complete map), this process is called the genome Gap closure (or fill hole).
Genome-wide optical mapping is a method of generating high-resolution, ordered whole-genome restriction digest maps using restriction endonuclease mapping of single-molecule DNA.
In 2010, OpGen introduced the Argus ™ workstation, an automated genome splicing and data analysis system based on patented optical mapping technology, that cleans high-quality whole-genome restriction endonucleases of the microbe genome under test within one business day Map. The system mainly consists of a Mapcard processing workstation, an optical scanning system, a data processing workstation and the like. The single-molecule DNA immobilized on the MapCard DNA surface is cut in situ by a restriction enzyme to keep the cut DNA fragments in sequence change. The DNA fragment was stained with fluorescent dye and then placed under a fluorescence microscope to collect the size and sequence information of each restriction enzyme fragment. The information was transformed and processed to generate the restriction enzyme site map of a single DNA molecule, Finally, a complete genome restriction endonuclease map was obtained by splicing the overlapping parts of all the DNA restriction endonuclease sites. The system was initially applied to the assembly of microbial genome sequences, comparative genomics and strain classification and has now been applied to the whole genome assembly of humans and animals and plants.
