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植物*2ELISA试剂盒,VD2 ELISA Kit ,英文缩写: VD2 ,规格:96T/48T ,产品别名: 植物*2(VD2)ELISA检测试剂盒, 植物*2(VD2)酶联免疫试剂盒
ELISA常用的方法主要包括双抗体夹心法、间接法、竞争法以及BAS-ELISA。
(1)植物*2ELISA试剂盒,VD2 ELISA Kit 双抗体夹心法是检测抗原zui常用的方法。夹心法利用两种一抗对目标抗原进行捕获和固定,在确保灵敏度的同时大大提高了反应的特异性,该方法的检测灵敏度可提高到pg级的水平。将已知抗体包被到固相表面,加入待检标本,标本中若含有相应抗原即与固相表面的抗体结合,洗涤去除未结合成分,加入该抗原特异的酶标记抗体,洗去未结合的酶标记抗体,加底物后显色。若标本中无相应抗原,固相表面即无抗原结合,加入的酶标记抗体则不能结合于固相并被洗涤去除,当加入无色底物后,因无酶催化故不显色。双抗体夹心法适用于测定2价或2价以上的大分子抗原,但不适用于测定半抗原及小分子单价抗原,因其不能形成两位点夹心。
(2)间接法是检测抗体zui常用的方法,其原理为利用酶标记的抗抗体以检测已与固相结合的受检抗体,故称为间接法。先将待测的蛋白包被在孔板内,然后依次加入一抗、酶标记u akn 的二抗和底物显色,通过仪器(例如酶标仪)定量检测抗原。这种方法操作简单但由于高背景而特异性较差,目前已逐渐被夹心法取代。间接法的优点是只要变换包被抗原就可利用同一酶标二抗建立检测相应抗体的方法。间接法成功的关键在于抗原的纯度。间接法中另一种干扰因素为正常血清中所含的高浓度的非特异性抗体。
(3)竞争法可用于测定抗原,也可用于测定抗体。当抗原材料中的干扰物质不易除去,或不易得到足够的纯化抗原时,可用此法检测特异性抗体。其原理为标本中的抗体一定量的酶标抗体竞争与固相抗原结合。标本中抗体量越多,结合在固相上的酶标抗体愈少,因此阳性反应呈色浅于阴性反应。如抗原为高纯度的,可直接包被于固相。如抗原中有干扰物质,直接包被不易成功,可采用捕获包被法,即先包被与固相抗原相应的抗体,然后加入抗原,形成固相抗原。洗涤除去抗原中的杂质,然后再加入标本和酶标抗体进行竞争结合反应。小分子抗原或半抗原因缺乏可用于夹心法的两个以上的位点,因此不能用双抗体夹心法进行测定,可以采用竞争法模式。其原理是标本中的抗原和一定量的酶标抗原竞争与固相抗体结合。标本中抗原量含量愈多,结合在固相上的酶标抗原愈少,zui后的显色也愈浅。
Chicken prolactin,PRL ELISA Kit X217622 鸡催乳素(PRL)ELISA Kit 96T/48T
Chicken Avian Leukosis Virus AG,ALV-AG ELISA Kit X217623 鸡白血病病毒抗原(ALV-AG)ELISA Kit 96T/48T
Chicken follicle-stimulating hormone,FSH ELISA Kit X217624 鸡促卵泡素(FSH)ELISA Kit 96T/48T
Chicken Luteinizing Hormone,LH ELISA Kit X217625 鸡促黄体激素(LH)ELISA Kit 96T/48T
Chicken Interferon γ ,IFN-γ ELISA Kit X217626 鸡γ干扰素(IFN-γ)ELISA Kit 96T/48T
Chicken Interleukin 6,IL-6 ELISA Kit X217627 鸡白介素6(IL-6)ELISA Kit 96T/48T
Chicken carbonic anhydrase,CA ELISA Kit X217628 鸡碳酸酐酶(CA)ELISA Kit 96T/48T
Chicken Anti-Thrombin Ⅲ,AT-Ⅲ ELISA Kit X217629 鸡抗*Ⅲ抗体(AT-Ⅲ)ELISA Kit 96T/48T
Chicken Heat Shock Protein 40,HSP-40 ELISA Kit X217630 鸡热休克蛋白40(HSP-40)ELISA Kit 96T/48T
Chicken Heat Shock Protein 20,HSP-20 ELISA Kit X217631 鸡热休克蛋白20(HSP-20)ELISA Kit 96T/48T
Chicken Heat Shock Protein 60,HSP-60 ELISA Kit X217632 鸡热休克蛋白60(Hsp-60)ELISA Kit 96T/48T
Chicken Interleukin 4,IL-4 ELISA Kit X217633 鸡白介素4(IL-4)ELISA Kit 96T/48T
Chicken Interleukin 2,IL-2 ELISA Kit X217634 鸡白介素2(IL-2)ELISA Kit 96T/48T
Chicken nitric oxide,NO ELISA Kit X217635 鸡血清一氧化氮(NO)ELISA Kit 96T/48T
Chicken Herpes simplexvirus Ⅱ antibody,HSVⅡ-Ab ELISA Kit X217636 鸡单纯疱疹病毒Ⅱ型抗体(HSVⅡ-Ab)ELISA Kit 96T/48T
Chicken Complement 3 split product,C3SP ELISA Kit X217637 鸡补体3裂解产物(C3SP)ELISA Kit 96T/48T
Chicken adrenomedullin,ADM ELISA Kit X217638 鸡肾上腺髓质素(ADM)ELISA Kit 96T/48T
Chicken Alternative macrophage activation-associated CC chemokine 1,AmAC-1 ELISA Kit X217639 鸡巨噬细胞替代激活相关化学因子1(AmAC-1)ELISA Kit 96T/48T
Chicken Immune RNA,Irna ELISA Kit X217640 鸡免疫核糖核酸(Irna)ELISA Kit 96T/48T
Chicken β-lactamase ELISA Kit X217641 鸡β内酰胺酶(β-lactamase)ELISA Kit 96T/48T
Chicken Xapanese Encephalitis IgG,XE IgG ELISA Kit X217642 鸡流行性乙型脑炎抗体IgG(XE IgG)ELISA Kit 96T/48T
Chicken α-Glucosidase ELISA Kit X217643 鸡α葡萄糖苷酶(α-glucosidase)ELISA Kit 96T/48T
Chicken Plaet Factor 4,PF-4 ELISA Kit X217644 鸡血小板因子4(PF-4/CXCL4)ELISA Kit 96T/48T
Chicken tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 ELISA Kit X217645 鸡肿瘤坏死因子相关凋亡诱导配体3(TRAIL-R3)ELISA Kit 96T/48T
Chicken L-Phenylalanine ammonla-lyase,PAL ELISA Kit X217646 鸡L苯*解氨酶(PAL)ELISA Kit 96T/48T
Chicken nicotinamide adenine dinucleotide phosphate,NADPH ELISA Kit X217647 鸡*腺嘌呤二核苷酸磷酸(NADPH)ELISA Kit 96T/48T
Chicken factor B,BF ELISA Kit X217648 鸡B因子(BF)ELISA Kit 96T/48T
Chicken 5-Nucleotidase,5-NT ELISA Kit X217649 鸡5核苷酸酶(5-NT)ELISA Kit 96T/48T
Chicken Laminin,LN ELISA Kit X217650 鸡层连蛋白/板层素(LN)ELISA KIT 96T/48T
Chicken 17-ketosteroids,17-KS ELISA Kit X217651 鸡17-酮类固醇(17-KS)ELISA Kit 96T/48T
Chicken N-acetyl-β-D-glucosaminida se,NAG ELISA Kit X217652 鸡N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit 96T/48T
Chicken glial fibrillary acidic protein,GFAP ELISA Kit X217653 鸡神经胶质纤维酸性蛋白(GFAP)ELISA Kit 96T/48T
Chicken Interleukin 17,IL-17 ELISA Kit X217654 鸡白介素17(IL-17)ELISA Kit 96T/48T
Chicken 1,3-βD glucosidase ELISA Kit X217655 鸡1,3-βD葡葡糖苷酶(1,3-βD glucosidase)ELISA Kit 96T/48T
Chicken soluble endothelial protein C receptor,sEPCR ELISA Kit X217656 鸡可溶性血管内皮细胞蛋白C受体(sEPCR)ELISA Kit 96T/48T
Rat soluble Toll-like receptor 6,sTLR-6 ELISA Kit X217657 大鼠可溶性Toll样受体6(sTLR6)ELISA Kit 96T/48T
Rat soluble Toll-like receptor 2,sTLR-2 ELISA Kit X217658 大鼠可溶性Toll样受体2(sTLR2)ELISA Kit 96T/48T
Rat Prostaglandin F2α,PGF2α ELISA Kit X217659 大鼠前列腺素F2α(PGF2α)ELISA Kit 96T/48T
Rat leukotriene E4,LT-E4 ELISA Kit X217660 大鼠白三烯E4(LTE4)ELISA Kit 96T/48T
Rat urokinase,UK ELISA Kit X217661 大鼠*(UK)ELISA Kit 96T/48T
Rat Apolipoprotein H,Apo-H ELISA Kit X217662 大鼠载脂蛋白H(Apo-H)ELISA Kit 96T/48T
Rat adrenomedullin,ADM ELISA Kit X217663 大鼠肾上腺髓质素(ADM)ELISA Kit 96T/48T
Rat Beta-Endorphin receptor,β-EPR ELISA Kit X217664 大鼠β内啡肽受体(β-EPR)ELISA Kit 96T/48T
Rat calcium/calmodulin-dependent protein kinase-Ⅱ,CAMK 2 ELISA Kit X217665 大鼠钙/钙调素依赖性蛋白激酶2(CAMK 2)ELISA Kit 96T/48T
Rat hepaticlipase,HL ELISA Kit X217666 大鼠肝脂酶(HL)ELISA Kit 96T/48T
Rat nicotinic acetylcholine receptor,N-AChR ELISA Kit X217667 大鼠烟碱型乙酰*受体(N-AChR)ELISA Kit 96T/48T
Rat muscarinic acetylcholine receptor,M-AChR ELISA Kit X217668 大鼠毒蕈碱型乙酰*受体(M-AChR)ELISA Kit 96T/48T
Rat activated protein C,APC ELISA Kit X217669 大鼠活化蛋白C(APC)ELISA Kit 96T/48T
Rat galanin,GAL ELISA Kit X217670 大鼠甘丙肽/甘丙素(GAL)ELISA Kit 96T/48T
Rat α-Glucosidase ELISA Kit X217671 大鼠α葡萄糖苷酶(α-glucosidase)ELISA Kit 96T/48T
Rat osteoclast differentiation factor,ODF ELISA Kit X217672 大鼠破骨细胞分化因子(ODF)ELISA Kit 96T/48T
Rat Toll-like receptor 9,TLR-9 ELISA kit X217673 大鼠Toll样受体9(TLR-9/CD289)ELISA kit 96T/48T
Rat Lebtospira IgG ELISA Kit X217674 大鼠钩端螺旋体IgG(Lebtospira)ELISA Kit 96T/48T
Rat Heat Shock Factor 1,HSF1 ELISA Kit X217675 大鼠热休克因子1(HSF1)ELISA Kit 96T/48T
Rat cytochrome P450,CYP450 ELISA Kit X217676 大鼠细胞色素P450(CYP450)ELISA Kit 96T/48T
Rat growth hormone releasing peptide-Ghrelin,GHRP-Ghrelin ELISA Kit X217677 大鼠生长激素释放肽ghrelin(GHRP-Ghrelin)ELISA Kit 96T/48T
Rat Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit X217678 大鼠内脏脂肪特异性*蛋白酶抑制剂(vaspin)ELISA Kit 96T/48T
Rat Bromodeoxyuridine,BrdU ELISA Kit X217679 大鼠溴脱氧核苷*(BrdU)ELISA Kit 96T/48T
Rat cytochrome P450 2E1,CYP2E1 ELISA Kit X217680 大鼠细胞色素P4502E1(CYP2E1)ELISA Kit 96T/48T
Rat α1-microglobulin,α1-MG ELISA Kit X217681 大鼠α1微球蛋白(α1-MG)ELISA Kit 96T/48T
Rat peroxisome proliferators activator receptors alpha,PPAR-α ELISA Kit X217682 大鼠过氧化物酶体增殖物激活受体α(PPAR-α)ELISA Kit 96T/48T
Rat calmodulin,CAM ELISA Kit X217683 大鼠钙调素(CAM)ELISA Kit 96T/48T
Rat Apolipoprotein E,Apo-E ELISA Kit X217684 大鼠载脂蛋白E(Apo-E)ELISA Kit 96T/48T
Rat orphanin FQ/nociceptin,OFQ/N ELISA Kit X217685 大鼠孤腓肽(OFQ/N)ELISA Kit 96T/48T
Rat Protein Phosphatase,PP ELISA Kit X217686 大鼠蛋白磷酸酶(PP)ELISA Kit 96T/48T
Rat thioredoxin reductase,TrxR ELISA Kit X217687 大鼠硫氧还蛋白还原酶(TrxR)ELISA Kit 96T/48T
Rat Thioredoxin,Trx ELISA Kit X217688 大鼠硫氧化还原蛋白(Trx)ELISA Kit 96T/48T
Rat Collagenase Type II ELISA Kit X217689 大鼠胶原酶II(Collagenase II)ELISA Kit 96T/48T
Rat Triggering Receptor Expresses on Myeloid Cells-1,TREM-1 ELISA Kit X217690 大鼠髓系细胞触发受体-1(TREM-1)ELISA Kit 96T/48T
Rat Collagenase I ELISA Kit X217691 大鼠胶原酶I(Collagenase I)ELISA Kit 96T/48T
Rat phospho Protein Kinase C,P-PKC ELISA Kit X217692 大鼠磷酸化蛋白激酶C(P-PKC)ELISA Kit 96T/48T
Rat Cathepsin K,cath-K ELISA Kit X217693 大鼠组织蛋白酶K(cath-K)ELISA Kit 96T/48T
Rat Alternative macrophage activation-associated CC chemokine 1,AMAC-1 ELISA Kit X217694 大鼠巨噬细胞替代激活相关化学因子1(AmAC-1)ELISA Kit 96T/48T
Rat bone morphogenetic proteins,BMPs ELISA Kit X217695 大鼠骨形成蛋白(BMPs)ELISA Kit 96T/48T
Rat Retinol binding protein 4,RBP-4 ELISA Kit X217696 大鼠*结合蛋白4(RBP-4)ELISA Kit 96T/48T
Rat 6-keto-prostaglandin F1a,6-K-PGF1a ELISA Kit X217697 大鼠6酮前列腺素F1a(6-keto-PGF1a)ELISA Kit 96T/48T
Rat Pentosidine ELISA Kit X217698 大鼠戊糖素(Pentosidine)ELISA Kit 96T/48T
Rat advanced glycation end products,AGEs ELISA Kit X217699 大鼠晚期糖基化终末产物(AGEs)ELISA Kit 96T/48T
Rat neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit X217700 大鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)ELISA Kit 96T/48T
Rat Helicobacter pylori IgG,Hp-IgG ELISA Kit X217701 大鼠幽门螺旋菌IgG(Hp-IgG)ELISA Kit 96T/48T
Rat Estrogen Receptor,ER ELISA Kit X217702 大鼠雌激素受体(ER)ELISA Kit 96T/48T
Rat β-Defensins ELISA Kit X217703 大鼠β-防御素(β-Defensins)ELISA Kit 96T/48T
Rat Dopamine D1 receptor,D1R ELISA Kit X217704 大鼠多巴胺D1受体(D1R)ELISA Kit 96T/48T
Rat adrenergic alpha-1A receptor,ADRA1A ELISA Kit X217705 大鼠肾上腺素能a1A受体(ADRA1A)ELISA Kit 96T/48T