【文献标题】MSC-ACE2 Ameliorates Streptococcus uberis-Induced Inflammatory Injury in MammaEpithelial Cells by UpregulatinIL-10/STAT3/SOCS3 Pathway

【作者】Shuping Yan, Chonghao Zhang, Xiaoxia Ji,et.al

【作者单位】南京农业大学（Nanjing Agricultural University）

【文献中引用产品】

小鼠白介素6(IL-6)ELISA试剂盒

小鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒

小鼠白介素1β (IL-1β)ELISA试剂盒

小鼠血管紧张素1-7（Ang 1-7) ELISA试剂盒

小鼠血管紧张素Ⅱ(ANG-Ⅱ)ELISA试剂盒

【关键词】S. uberis, MSC-ACE2, inflammatory injury, pyroptosis, blood-milk barrier, mammary epithelial cells

【DOI】doi: 10.3389/fimmu.2022.870780

【影响因子(IF)】8.78

【出版期刊】《Frontiers in Immunolog》

【产品原文引用】

Analyzing the Concentration of IL-6,TNF-a, IL-Ib, Ang-(1–7), Ang II, and NAGase Activity

In the supernatant after cell culture, IL-6, TNF-a, IL-Ib,Ang-(1–7), and Ang II concentrations were measured by ELISA (Hengyuan Biotechnology Co., Shanghai, China). The manufacturer’s instructions were followed for the ELISA test. As a unit of measurement, TNF-a, IL-Ib, Ang-(1–7), and Ang II were demonstrated as ng/L. In this case, IL-6 was demonstrated as pg/mL. Using the instructions included in the kit purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China), the action of NAGase was determined in the supernatant of the cell culture medium. NAGase activity was measured in units of U/L.

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