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化学发光成像系统也是专业为化学发光成像的探测而设计的化学发光成像仪,从凝胶或杂交信号的采集所需凝胶荧光图像,凝胶化学发光图像和凝胶色度图像,*的凝胶成像系统的设计满足您的所有凝胶印迹成像,DNA荧光成像和蛋白质荧光成像应用需要。使用高量子效率的制冷型CCD相机,超大光圈高性能的镜头,为化学发光图像采集和分析提供强大的硬件技术保证。
化学发光成像系统具有*的照明和成像技术,配备600百万高性能制冷型CCD相机和密封的暗室,可连续采集凝胶荧光图像长达90分钟。
化学发光成像系统非常适合从Western blot ECL图像中采集化学发光,获取化学发光图像,还设有各种图像的光源的设置允许蛋白质2D DIGE成像。
化学发光成像系统还可以使用不同颜色光源如紫红色,绿色,蓝色和白色的光激发荧光采集图像.
化学发光成像系统特色
1、高分辨率和高灵敏度600万象素制冷型CCD相机,制冷温度高达-55-5摄氏度
2.16-bit、高动态范围、高达6×6 Bin Mode
3.four-level移动平台具有自动对焦功能
4可调RGB光强获得图像
5、用户界面上的冷却状态指示
6。自动备份防止重要数据丢失
7、通过局域网登录的远程操作
化学发光成像系统应用
化学发光成像结果
Western blot Western blot
化学发光法 ECL探测 化学发光ECL +(NG Fg水平)检测
曝光时间:30分钟 曝光时间:3分钟
30分钟的曝光时间后没有观察到明显的噪声。 THP1细胞线样品从7.5微克到58.6纳克(2倍稀释)的制备和加载SDS-PAGE。
箭头表示蛋白质样本为58.6纳克。 蛋白质转移到硝酸纤维素膜上进行进一步的免疫印迹分析。ECL +(NG Fg水平)试剂盒应用于膜。
箭头表示蛋白质样本的120 ng。
THP1细胞线样品从15微克到120纳克(5倍稀释)
的制备和加载SDS-PAGE。蛋白质转移到硝酸纤
维素膜上进行进一步的免疫印迹分析。
ECL底物试剂盒应用于膜。
Colorimetric应用结果
Western Blot SDS-PAGE
HRP/DAB treatment coomassie blue treatment
Exposure time: 30ms exposure time : 30ms
Epi White Light Setting White Backlight setting
荧光成像应用结果
DNA Agarose Gel EtBr Stain UV light Excitation Lower Level 200 ms | DNA Agarose Gel ECO Safe Stain Epi Blue Light Excitation Mid Level 200 ms |
Chemlux 600 W | Chemlux 600 BW% | Chemlux 600 RGBW* | ||
Control Panel | Display | 10.4" Multi-touch LCD Panel | 10.4" Multi-touch LCD Panel | 10.4" Multi-touch LCD Panel |
Touch Panel | Cap Sense Touch Panel | Cap Sense Touch Panel | Cap Sense Touch Panel | |
OS | Embedded Linux | Embedded Linux | Embedded Linux | |
Camera | Sensor | 6M CCD | 6M CCD | 6M CCD |
Resolution | 2,736 X 2,160 Pixels | 2,736 X 2,160 Pixels | 2,736 X 2,160 Pixels | |
Pixel Size | 4.54 mm x 4.54 mm | 4.54 mm x 4.54 mm | 4.54 mm x 4.54 mm | |
Exposure time | 10 ms – 90 min | 10 ms – 90 min | 10 ms – 90 min | |
Cooling Temperature | -550C ± 50C from Ambient | -550C ± 50C from Ambient | -550C ± 50C from Ambient | |
Optical | Lens | 1" 25 mm Fixed Focal Length | 1" 25 mm Fixed Focal Length | 1" 25 mm Fixed Focal Length |
Auto Focus | Yes (Auto) | Yes (Auto) | Yes (Auto) | |
IRIS | F0.95 | F0.95 | F0.95 | |
Zoom | 3- stage position | 3- stage position | 3- stage position | |
1. 12 cm x 9 cm | 1. 12 cm x 9 cm | 1. 12 cm x 9 cm | ||
2. 19 cm x 14 cm | 2. 19 cm x 14 cm | 2. 19 cm x 14 cm | ||
3. 26 cm x 20 cm | 3. 26 cm x 20 cm | 3. 26 cm x 20 cm | ||
Manual operation | Manual operation | Manual operation | ||
Filter | 6 Position (Motor-powered) | 6 Position (Motor-powered) | 6 Position (Motor-powered) | |
Viewing Area | 26 cm x 20 cm (Maximum) | 26 cm x 20 cm (Maximum) | 26 cm x 20 cm (Maximum) | |
Light Source | UV@ | 306 nm UV Trans-illuminator (For Chemlux 600 W-UV only) | 306 nm UV Trans-illuminator (For Chemlux 600 BW-UV only) | 306 nm UV Trans-illuminator (For Chemlux 600 RGBW-UV only) |
White Backlight Plate | Yes, 26 cm x 20 cm | Yes, 26 cm x 20 cm | Yes, 26 cm x 20 cm | |
EPI RGB | N/A | EPI Blue light | EPI R/G/B light | |
EPI White Light | Yes | Yes | Yes | |
Lift Plate | 3 Lift Level | Manual Adjustable | Manual Adjustable | Manual Adjustable |
Connection | USB 2.0 Port | X3 (Front x1; Rear x2) | X3 (Front x1; Rear x2) | X3 (Front x1; Rear x2) |
10/100 Ethernet Port | X1 | X1 | X1 | |
Capture | Capture Mode | UV (UV Series only) | UV (UV Series only) | UV (UV Series only) |
White backlight | White backlight | White backlight | ||
N/A | EPI blue | EPI R/G/B light | ||
Chemiluminescent (Dark Room) | Chemiluminescent (Dark Room) | Chemiluminescent (Dark Room) | ||
EPI white | EPI white | EPI white | ||
Image Format | TIFF 16 Bit/ JPG/BMP | TIFF 16 Bit/ JPG/BMP | TIFF 16 Bit/ JPG/BMP | |
Storage | 8 GB Data storage | 8 GB Data storage | 8 GB Data storage | |
Certificates | CE & FCC | CE & FCC Certified | CE & FCC Certified | CE & FCC Certified |
Dimensions | L x W x H | 452 X 423 X 659 mm | 452 X 423 X 659 mm | 452 X 423 X 659 mm |
Power | Input | AC100-240V, 50/60Hz, 200W | AC100-240V, 50/60Hz, 200W | AC100-240V, 50/60Hz, 200W |
Weight | Net Weight | 52 Kg (Approximately) | 52 Kg (Approximately) | 52 Kg (Approximately) |