12-HETE Hypertension ELISA背景：

This competitive ELISA kit is for determination of 12-HETE (12-Hydroxyeicosatetraenoic acid) levels in biological samples. The specificity of the 12-HETE ELISA was investigated using authentic 12-HETE and fatty acids which, based on their structure, might be anticipated to compete with 12-HETE for binding to antibodies against 12-HETE.

HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxyl product produced from the reduction of 12(S)-hydroperoxytetraenoic eicosatetraenoic acid [12(S)-HpETE], which is itself is a 12-lipoxygenase metabolite of arachidonic acid. It has recently been reported that platelet 12(S)-HETE production is enhanced in the spontaneously hypertensive rat1. 12(S)-HETE levels and 12-lipoxygenase (12-LO) protein are increased in patients with essential hypertension2, also suggesting a role for this

metabolite in human hypertension. These metabolites exhibit a variety of biological activities such as mediation of angiotensin II–induced intracellular calcium transients in cultured rat vascular smooth muscle cells3 and as a second messenger in angiotensin-II induced aldosterone production4

. 12(S)-HETE also acts as an aggregator of polymorphonuclear leukocytes5 and is a highly selective ligand used to label mu opioid receptors6 and serves as a biomarker of Churg-Strauss syndrome7.

Each kit can be used for triplicate analyses of up to 24 samples contains using a 96 well plate format, and contains a vial of 12-HETE standard, a vial of 12-HETE-conjugated horseradish peroxidase (HRP), and buffers for sample and HRP dilutions, and plate washing.

这种竞争性ELISA试剂盒用于测定生物中12-HETE（12-羟基二十碳四烯酸）的水平样品。使用真实的12-HETE和脂肪酸研究12-HETE ELISA的特异性可以预期在其结构上与12-HETE竞争与针对12-HETE的抗体的结合。抗-12-HETE仅与15-HETE和13-HODE轻微交叉反应。

HETE是花生四烯酸通过脂氧合酶代谢产生的副产物。

12（S）-HETE是12-（S）-氢过氧四烯酸二十碳四烯酸还原产生的立体特异性羟基产物[12（S）-HpETE]，其本身是花生四烯酸的12-脂氧合酶代谢产物。最近有报道称自发性高血压大鼠血小板12（S）-HETE的产生增强1.12（S）-HETE水平和12-原发性高血压患者脂氧合酶（12-LO）蛋白增加2，也暗示了这方面的作用人类高血压的代谢产物。这些代谢产物表现出多种生物活性，如介导血管紧张素II诱导的培养大鼠血管平滑肌细胞内钙瞬变3作为第二个血管紧张素II诱导的醛固酮产生中的信使4.12（S）-HETE还充当多形核白细胞5是一种用于标记μ阿片受体的高选择性配体6并作为丘格-斯特劳斯综合征的生物标志物7.

每个试剂盒可用于对多达24个样品进行一式三份的分析，包含使用96孔板格式的样品，并包含一个小瓶12-HETE标准，一小瓶12-HETE缀合的辣根过氧化物酶（HRP），以及用于样品和HRP的缓冲液稀释和板洗涤。

艾美捷12-HETE Hypertension ELISA#DRD-12H1参考文献：

1. Stern N, Kisch ES, Knoll E. Platelet lipoxygenase in spontaneously hypertensive rats. Hypertension. 1996;27:1149–1152.

2. González-Núñez, Daniel, Joan Claria, Francisca Rivera, Esteban Poch. Increased levels of 12(S)-HETE in patients with essential hypertension. Hypertension 2001; 37: 334-338

3. Natarajan, Rama, Noe Gonzales, Linda Lanting, Jerry Nadler. Role of the Lipoxygenase pathway in angiotensin II-induced vascular smooth muscle cell hypertrophy. Hypertension 1994; 23: I142

4. J L Nadler, R Natarajan and N Stern. Specific action of the lipoxygenase pathway in mediating angiotensin II-induced aldosterone synthesis in isolated adrenal glomerulosa cells. J Clin Invest. 1987;80(6):1763–1769.

5. O'Flaherty JT, Thomas MJ, Lees CJ, McCall CE Neutrophil-aggregating activity of monohydroxyeicosatetraenoic acids.Am J Pathol. 1981 Jul;104(1):55-62.

6. Christie, M.J., C.W. Vaughan and S.L. Ingram. Opioids, NSAIDs, and 5-lipoxygenase inhibitors act synergistically in brain via arachidonic acid metabolism. Inflamm. Res. 1999, 48: 1-4.

7. Szczeklik W, Sanak M, Mastalerz L, Sokołowska BM, Gielicz A, Soja J, Kumik J, Musiał J, Szczeklik A.12-hydroxy-eicosatetraenoic acid (12-HETE): a biomarker of Churg-Strauss syndrome. Clin Exp Allergy. 2012 Apr;42(4):513-22

12-HETE Hypertension ELISA相关研究：

15-HETE Hypertension ELISA Kit 15H1