艾美捷MethylFlash 5-mC RNA甲基化定量分析试剂盒（荧光检测）是一套完整的优化缓冲液和试剂，用于荧光定量总5-甲基胞嘧啶（5-mC）RNA甲基化水平。 将 RNA结合至 strip-wells，使用 5-mC RNA 辨认抗体与侦测抗体然后进行荧光成色反应，利用荧光光谱进行荧光强度测定甲基化修饰换算。

艾美捷MethylFlash 5-mC RNA甲基化定量分析试剂盒 (Fluorometric)包含 5-mC RNA 甲基化定量实验所需的所有最佳化试剂，可以使用任何物种的 total RNA 样品来进行实验，如哺乳动物、植物、真菌、细菌和病毒等，无论是从培养细胞、新鲜或冷冻的组织、血浆/血清或体液等来源皆可适用。  

艾美捷MethylFlash 5-mC RNA甲基化定量分析试剂盒特点：

快速 – 全部反应只需2小时40分

高效 – 稳定的品质让您的数据拥有高度重复性

方便 – 流程简单快速

高敏感性 – 可从200 ng的RNA样品侦测到至低0.02%的5-mC RNA 修饰

专一性 – 高专一性抗体使用, 不会有其他unmethylated cytosine或 hydroxymethylated cytosine的交互辨识干扰

均一性 – 套组中皆附有positive和negative control

准确性 – 极-佳的positive control设计可让您的实验数据达到如HPLC-MS分析实验的效能

弹性化 – 採用strip-well的microplate格式, 能依照实验需求来进行少量或是高通量实验

5-mC RNA甲基化定量分析试剂盒引文赏析：

Betlej G et. al. (October 2022). RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines. J Bone Oncol. 36:100448.

Bataglia L et. al. (July 2021). Active genic machinery for epigenetic RNA modifications in bees. Insect Mol Biol.

Park MH et. al. (January 2020). Mono-(2-Ethylhexyl)phthalate Regulates Cholesterol Efflux via MicroRNAs Regulated m⁶A RNA Methylation. Chem Res Toxicol.

Lewinska A et. al. (August 2017). Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression. Redox Biol. 14:20-34.

Lewinska A et. al. (February 2017). Downregulation of Methyltransferase Dnmt2 Results in Condition-dependent Telomere Shortening and Senescence or Apoptosis in Mouse Fibroblasts. J Cell Physiol.