FITC-CM-polysucroses are standard FITC-polysucrose carrying carboxymethyl-substituents. Polysucrose (renamed from Ficoll™) is a polymer which displays excellent biocompatibility. The CM (carboxymethyl) group gives the product anionic (negative) charge. All batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produce FITC-CM-polysucroses with a molecular weight of 70 kDa and 400 kDa. FITC-CM-polysucrose is supplied as a yellow powder.

Structure

Polysucrose is a polymer synthesised by cross-linking sucrose with epichlorohydrin. FITC-CM-polysucroses are manufactured by reacting selected polysucrose fractions with an activated carboxymethyl derivative in alkali whereby O-carboxymethyl groups are introduced along the polysucrose chain. The carboxyl content is approximately 5% which is equivalent to about one CM group for every five glucose units. Thereafter, fluorescein (FITC) groups are introduced by reaction with fluorescein isothiocyanate. Polysucrose has a more spherical structure and is less flexible compared to dextran.

Spectral data

FITC-polysucrose has an excitation maximum of 495 and an emission maximum at 517 nm at pH 9.

Storage and stability

FITC-CM-polysucrose is stable for more than 6 years when stored dry in well-sealed containers at ambient temperature.

Solubility

FITC-CM-polysucrose dissolves readily in water.

Application

FITC-CM-polysucrose are mostly used for studies of permeability and microcirculation. The carboxyl groups will impart an overall negative charge to the molecule, which may be valuable in gaining information on the permeability characteristics of cell membranes and tissues. FITC-CM-polysucrose has played an interesting role in elucidating the properties of the glomerular membrane.

艾美捷CM-polysucrose（CM-聚蔗糖，羧甲基-聚蔗糖，Carboxymethyl-polysucrose）#CMP70：

FITC-CM-聚蔗糖是携带羧甲基取代基的标准FITC-聚蔗糖。聚蔗糖（从Ficoll™更名）是一种具有优异生物相容性的聚合物。CM（羧甲基）基团赋予产物阴离子（负）电荷。检查所有批次的分子量、取代度、干燥失重和游离 FITC。TdB实验室生产分子量为70 kDa和400 kDa的FITC-CM-聚蔗糖。FITC-CM-聚蔗糖以黄色粉末形式提供。

结构：

聚蔗糖是通过蔗糖与环氧氯丙烷交联合成的聚合物。FITC-CM-聚蔗糖是通过将选定的聚蔗糖级分与碱中的活化羧甲基衍生物反应来制造的，从而沿聚蔗糖链引入O-羧甲基。羧基含量约为5%，相当于每五个葡萄糖单位约一个CM基团。此后，通过与异硫氰酸荧光素反应引入荧光素（FITC）基团。与葡聚糖相比，聚蔗糖具有更球形的结构，并且灵活性较差。

光谱数据：

FITC-聚蔗糖在pH 495时的激发最大值为517，在9 nm处的发射最大值。

储存和稳定性

FITC-CM-polysucrose在环境温度下干燥储存在密封良好的容器中时可稳定保存 6 年以上。

溶解性

FITC-CM-polysucrose易溶于水。

FITC-CM-polysucrose的应用：

主要用于渗透性和微循环的研究。羧基将赋予分子整体负电荷，这对于获得有关细胞膜和组织的通透性特征的信息可能是有价值的。FITC-CM-聚蔗糖在阐明肾小球膜的性质方面发挥了有趣的作用。