中英文说明书丨艾美捷 RmesFect CRISPR转染试剂
时间:2023-04-03 阅读:102
RmesFect™ CRISPR transfection Reagent based on the TEE-technology is specifically designed for mRNA/gRNA transfection with high efficiency and low toxicity. RmesFect™ is efficient in a large variety of cells.
Benefits:
Highly efficient: achieve RNAs delivery in all cells
Ready-to-use: no need for additional buffer
Low nucleic acid amount : minimized toxicity
Protects RNAs against degradation
Compatible with any culture medium
Serum compatible : Medium changed not required
mRNA transfection provides several advantages over plasmid DNA delivery :
No need for nuclear uptake. mRNA translation into proteins occurs in the cytoplasm.
Faster protein expression than DNA transfection
No genomic integration
Protein expression in a total promoter-independent manner
Additional products for CRISPR Cas9 experiments:
PolyMag CRISPR for Genome editing using expression plasmids
ProDeliverIN CRISPR for Cas9 protein delivery
ViroMag CRISPR to enhance transduction efficiency of CRISPR/Cas9 viruses
基于TEE技术的艾美捷 RmesFect™ CRISPR转染试剂专为mRNA/gRNA转染而设计,具有高效率和低毒性的特点。RmesFect™在多种细胞中都是有效的。
OZ Biosciences RmesFect CRISPR转染试剂特点:
高效:在所有细胞中实现RNA递送
即用型:无需额外的缓冲液
低核酸量:毒性最小化
保护 RNA 免受降解
与任何培养基兼容
血清兼容:无需更换培养基
图 1:根据标准方案,使用 RmesFect CRISPR 转染编码 GFP 和 RFP(各 24.0μg)的 mRNA 转染 DC25 细胞。转染后24h在显微镜荧光下分析图像。
与质粒DNA递送相比,mRNA转染具有以下优势:
无需核吸收。mRNA翻译成蛋白质发生在细胞质中。
蛋白表达速度快于DNA转染
无基因组整合
以完-全启动子非依赖的方式表达蛋白质
用于CRISPR Cas9实验的其他产品:
PolyMag CRISPR用于使用表达质粒进行基因组编辑
ProDeliverIN CRISPR用于 Cas9 蛋白递送
ViroMag CRISPR提高CRISPR/Cas9病毒的转导效率