人黏膜地址素细胞黏附分子(MAdCAM-1)elisa北京报价

中文名称：人黏膜地址素细胞黏附分子(MAdCAM-1)

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本试剂盒应用双抗体夹心法测定标本中人黏膜地址素细胞黏附分子(MAdCAM-1)水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入，再与HRP标记的结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中的浓度。

试剂的准备按试剂盒说明书的要求准备实验中需用的试剂。ELISA中用的蒸馏水或去离子水，包括用于洗涤的，应为新鲜的和高质量的。自配的缓冲液应用pH计测量较正。从冰箱中取出的试验用试剂应待温度与室温平衡后使用。试剂盒中本次试验不需用的部分应及时放回冰箱保存。

The E-Z 96? Mag-Bind? Tissue RNA Kit provides a novel technology for total RNA isolation from plant and animal tissues. This kit allows the rapid and reliable isolation of high-quality total cellular RNA from a wide variety of tissues. Samples are first lysed in a specially formulated buffer and then proteins and DNA are precipitated. The Supertanent is combined with magentic beads and after a couple of quick wash steps pure RNA is eluted. Unlike column-based systems, the binding of nucleic acids to magnetic partciles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are also completly re-suspended during the wash steps of the purification protocol, which enhances contact with the wash buffer and removal of contaminants and increasing nucleic acid purity. E.Z.N.A.? Mag-Bind? Tissue RNA procedure can be automated with most robotic workstations。

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