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猪透明质酸(HA))检测试剂盒需要但未提供的材料
1.可在450nm处进行读数的酶标仪;
2.能精确吸取10-200μL的移液器;
3.可调多道(8)移液器(50-200μL);
4.供可调多道移液器使用的试剂存放槽;
5.洗瓶或洗板机;
6.蒸馏水或去离子水;
7.1升的圆柱形量筒;
8.移液器:1和(10或25 mL);
9.移液器枪头;
10.纸巾;
11.计时器,用以监控温育步骤;
12.处理池和消毒剂(例如:0.5% 次氯酸钠, 10% 家用漂白水);
猪透明质酸(HA))检测试剂盒试验以灵敏度较高、特异性较好的特点在临床上得到了广泛的应用,但操作中的各个环节对试验的检测效果影响较大,如不注意,有可能导致显色不全、花板等结果。我将操作中各个环节常出现问题的原因及解决办法总结于下,以期给同行带来一些启发,提高试验质量。
猪透明质酸(HA))检测试剂盒的洗涤一般可采用以下方法:
②将洗涤液注满板孔;
④吸干孔内液,也可倾去液体后在吸水纸上拍干。洗涤的次数一般为3~4次,有时甚至需洗5~6次
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