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Gibco Sf-900 Medium (1.3X), liquid ,货号: 10967-032 ,包装: 100ml ,品牌: Gibco
Gibco Sf-900 Medium (1.3X), liquid详细描述:
Form: Liquid
Brand: Sf-900™, GIBCO®
Species: Spodoptera frugiperda
Cell Line: Sf9, Sf21
Cell Type: Insect Cells
Glutamine: L-Glutamine
Product Size: 100 ml
Gibco Sf-900 III SFM (1X),liquid 12658-019 500ml Gibco
Sf-900™ III SFM is a low-hydrolysate, protein-free, animal origin-free medium that provides consistency, and reliability in cell growth. Because this medium does not include animal-derived components, the need to screen for adventitious agents is reduced.
Optimized to support and maximize Sf9 and Sf21 cell growth and recombinant gene expression in suspension culture using both baculovirus and stable insect expression systems Supports Sf9 cell growth to densities of 10 to 14 x 106 cells/ml Protein-free, low-hydrolysate formulation improves lot-to-lot consistency and reliability Contains no components of animal origin,facilitating the regulatory process
Storage:Store in the dark at 2 to 8°C.
Gibco Sf-900 III SFM (1X),liquid详细描述:
Form: Liquid
Brand: Sf-900™, GIBCO®
Species: Spodoptera frugiperda
Cell Line: Sf9, Sf21
Glutamine: L-Glutamine
Serum Level: Serum-Free
Product Size: 500 ml
Classification: Protein-Free, Animal Origin-Free, Serum-Free
更多GIBCO血清产品
保存血清的方法:应保存在-5度至-20度。然而,若存放于4度时,请勿超过一个月。若您一次无法用完一瓶,建议将无菌分装血清至恰当的灭菌容器内,再放回冷冻。
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如何解冻血清才不会使产品质量受损?将血清从冷冻箱取出后,先置于2-8度冰箱使之溶解,然后在室温下使之全溶。但必须注意的是,溶解过程中必须规则地摇晃均匀。
何谓热灭活?一般是以56度,30分钟来处理已解冻的血清,因为此加热步骤,可以使补体去活化,而补体所参与的反应有:溶细胞活性,平滑肌的收缩,肥大细胞和血小板组胺的释放,增强吞噬作用,淋巴细胞、巨噬细胞的趋化和激活。
有必要做热灭活吗?实验显示,经过正确处理的热灭活血清,对大多数的细胞而言是不需要的。经此处理过的血清对细胞的生长只有微小的促进,或*没有任何作用,甚至通常因为高温处理影响了血清的质量,而造成细胞生长速率的降低。而经过热处理的血清,沉淀物的形成会显著的增多,这些沉淀物在倒立显微镜下观察,像是“小黑点”,常常会让研究者误以为是血清遭受污染,而把血清放在37度环境中,又会使此沉淀物更增多,使研究者误认为是微生物的分裂扩增。
因此我们建议大家,热灭活若非必须,您可以不需要做热处理这一步。如此一来,不但节省您宝贵的时间,更确保您血清的质量!另外,购买已灭活成品也不失一个捷径,请在购买的时候注意询问厂家是否是已灭活血清。