Anti-CD34抗体，BD抗体总代理
时间：2014-06-05 阅读：998
Rabbit Anti-CD34抗体，BD抗体总代理 antibody
FROM:BD
Catalog Number :RS-90512
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ） background:
Hematopoietic Stem Cell Marker
Endothelial Markers
Tumor biomarkers
The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Specificity :
–Anti-CD34 is a rabbit polyclonal antibody
–specific for CD34 of mouse,rat
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
–Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 39 kDa
Application :
- ELISA=1:500-1000
- IHC-P=1:100-500
- IHC-F=1:100-500
- IF=1:150-200
– not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.Anti-CD34抗体，BD抗体总代理