Anti-Brdu ,FITC conjugated

Catalog Number : Rs-0489R-FITC

Quantity size : 0.1ml （2mg/ml; Reconstitute with 0.25 ml of sterile distilled water.）

The immunocytochemical detection of bromodeoxyuridine （BrdU） incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

pecificity :   

· Anti-Brdu is a rabbit polyclonal antibody, FITC conjugated              

specific fo Brdu of  Human, Mouse, Rat, Dog, Horse

· Protein G affinity chromatography purification, purity :>95%

· Isotype:  IgG

· mol wt:  48 kDa

Application :  

· FCM: 1:20-100

· Immunohistochemistry : 1:20-200

· Immunocytochemistry: 1:20-200

· Excitation spectrum: 490-495nm

· Emission spectrum: 520-530nm

· Optimal working dilutions must be determined by the end user.

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.