Rabbit  Anti-Skp2 p45 
Catalog Number :  HZ773961       Quantity size :   0.1ml  (dilute with pH 7.4    0.01 M PBS or antibody diluent ) 
Background:  The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.
pecificity :    
–Anti-Skp2 p45 is a rabbit polyclonal antibody unconjugated                 
–specific for Skp2 p45 of human, mouse, rat            
–use for western blotting,elisa,immunoprecipitation and immunohistochemistry
–Protein G affinity chromatography purification, purity :>95%
–Isotype:  IgG
–mol wt:  45kDa 
Application :   
–Western blotting 1:100-500                 
–Immunohistochemistry 1:100-500                  
–ELISA 1:500-1000                   
–Optimal working dilutions must  be determined by the end user.
Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃
 Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.