起订量:
ELISA是以免疫学反应为基础,将抗原、抗体体的特异性反应与酶对底物的高效催化作用相结合起来的一种敏感性很高的试验技术。
小鼠糖原磷酸化酶同工酶II GP-II是北京方程生物公司的优势产品,2017年买小鼠糖原磷酸化酶同工酶II GP-II送好礼*活动火热进行中......
Pig Histone H4 (H4) ELISA
contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the test. History Before the development of the ELISA, the only option for conducting an immunoassay was radioimmunoassay, a technique using radioactively-labeled antigens or antibodies. In radioimmunoassay, the radioactivity provides the signal, which indicates whether a specific antigen or antibody is present in the sample. Radioimmunoassay was first described in a paper by Rosalyn Sussman Yalow and Solomon Berson published in 1960.[5] Because radioactivity poses a potential health threat, a safer alternative was sought. A suitable alternative to radioimmunoassay would substitute a non-radioactive signal in place of the radioactive signal. When enzymes (such as peroxidase) react with appropriate substrates (such as ABTS or 3,3’,5,5’-Tetramethylbenzidine), a change in color occurs, which is used as a signal. However, the signal has to be associated with the presence of antibody or antigen, which is why the enzyme has to be linked to an appropriate antibody. This linking process was independently developed by Stratis Avrameas and G.B. Pierce.[6] Since it is necessary to remove any unbound antibody or antigen by washing, the antibody or antigen has to be fixed to the surface of the container; i.e., the immunosorbent has to be prepared. A technique to accomplish this was published by Wide and Jerker Porath in 1966.[7] In 1971, Peter Perlmann and Eva Engvall at Stockholm University in Sweden, and Anton Schuurs and Bauke van Weemen in The Netherlands independently published papers that synthesized this knowledge into methods to perform EIA/ELISA.[8][9]
本试剂盒采用双抗体夹心酶联免疫吸附法定量检测小鼠TNF-α。小鼠TNF-α试剂盒仅供科学研究,不用于临床诊断。肿瘤坏死因子 α (TNF-α) 也称为恶病质素和 TNFSF1A, 是一种脂肪因子,参与全身的炎症,同时也是刺激急性期反应的细胞因子之一。 它主要由活化的巨噬细胞产生, 也可由其它类型的细胞分泌,如 CD4+ 淋巴细胞、 NK 细胞、 中性粒细胞、 肥大细胞、嗜酸性粒细胞和神经元等。 它在免疫应答中具有多种调节功能, 还可作为潜在的热原。 TNF-α 对刺激物 (感染因子或组织受损) 产生应答后,在全身循环,激活中性粒细胞,改变血管内皮细胞的特性, 调控其它组织的代谢活性,以及通过诱导局部凝血作用展现肿瘤杀伤活性。 TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。 zui近,越来越多的信息表明 TNF-α 也参与了 AIDS 的发病机制。 TNF-α 的测定对于移植研究也非常有用。
猪红细胞生成素(EPO)检测盒Pig Erythropoietin (EPO) ELISA
猪甲硫腺苷磷酸化酶(MTAP)检测盒Pig Methylthioadenosine Phosphorylase (MTAP) ELISA
猪外核苷酸焦磷酸酶/磷酸二酯酶2(ENPP2)检测盒Pig Ectonucleotide Pyrophosphatase/Phosphodiesterase 2 (ENPP2) ELISA
人泛素羧基端酯酶L1(UCHL1)测定盒Human Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) ELISA
猪中性粒细胞明胶酶关联脂质运载蛋白(NGAL)检测盒Pig Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA
人心肌肌钙蛋白I(TNNI3)测定盒Human Troponin I Type 3, Cardiac (TNNI3) ELISA
人白介素18结合蛋白(IL18BP)测定盒Human Interleukin 18 Binding Protein (IL18BP) ELISA
猪抵抗素(RETN)检测盒Pig Resistin (RETN) ELISA
猪花生四烯酸(AA)检测盒Pig Arachidonic Acid (AA) ELISA
人骨成型蛋白10(BMP10)测定盒Human Bone Morphogenetic Protein 10 (BMP10) ELISA
人HtrA*肽酶4(HTRA4)测定盒Human HtrA Serine Peptidase 4 (HTRA4) ELISA
人白介素17B(IL17B)测定盒Human Interleukin 17B (IL17B) ELISA