小鼠信号转导分子 Smad3代检测

试剂盒名称:小鼠信号转导分子 Smad3代检测

Pig Interleukin 15 (IL15) ELISA

规格: 96T/48T
品牌:BIOFINE
种属:人ELISA试剂盒
检测波长:450nm
所需样本体积: 50-100ul
适用范围:仅供科研
保存及有效期:2-8℃，六个月，-20℃一年
检测目的:用于测定血清，血浆及相关液体小鼠信号转导分子 Smad3含量。适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本。 

ELISA results using S-OIV A neuraminidase antibody at 1 μg/ml to probe the immunogenic and the corresponding seasonal influenza A neuraminidase peptides at 50, 10, 2, and 0 ng/ml. Because the ELISA can be performed to evaluate either the presence of antigen or the presence of antibody in a sample, it is a useful tool for determining serum antibody concentrations (such as with the HIV test[3] or West Nile Virus). It has also found applications in the food industry in detecting potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.[4] ELISA can also be used in toxicology as a rapid presumptive screen for certain classes of drugs. The ELISA was the first screening test widely used for HIV because of its high sensitivity. In an ELISA, a person's serum is diluted 400-fold and applied to a plate to which HIV antigens are attached. If antibodies to HIV are present in the serum, they may bind to these HIV antigens. The plate is then washed to remove all other components of the serum. A specially prepared "secondary antibody" — an antibody that binds to other antibodies — is then applied to the plate, followed by another wash. This secondary antibody is chemically linked in advance to an enzyme. Thus, the plate will contain enzyme in proportion to the amount of secondary antibody bound to the plate. A substrate for the enzyme is applied, and catalysis by the enzyme leads to a change in color or fluorescence. ELISA results are reported as a number; the most controversial aspect of this test is determining the "cut-off" point between a positive and a negative result. A cut-off point may be determined by comparing it with a known standard. If an ELISA test is used for drug screening at workplace, a cut-off concentration, 50 ng/mL, for example, is established, and a sample that contains the standard concentration of analyte will be prepared. Unknowns that generate a signal that is stronger than the known sample are "positive." Those that generate weaker signal are "negative." Doctor Dennis E Bidwell and Alister Voller created the test.

小鼠信号转导分子 Smad3结果判定：试验有效性：阳性对照孔平均值≥1.00; 阴性对照平均值≤0.10临界值（CUT OFF）计算：临界值=阴性对照孔平均值+0.15阴性判定： 样品 OD 值< 临界值 （CUT OFF） 者为人 EB 病毒 Rta 蛋白抗体 IgG(Rta-IgG)阴性阳性判定： 样品 OD 值≥ 临界值 （CUT OFF） 者为人 EB 病毒 Rta 蛋白抗体 IgG(Rta-IgG)阳性注意事项1．操作严格按照说明书进行，本试剂不同批号组分不得混用。2．试剂盒从冷藏环境中取出应在室温平衡 15-30 分钟后方可使用，酶标包被板开封后如未用完，板条应装入密封袋中保存。3．浓洗涤液可能会有结晶析出，稀释时可在水浴中加温助溶，洗涤时不影响结果。4． 封板膜只限一次性使用，以避免交叉污染。

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