调制叶绿素荧光成像系统——IMAGING-PAM

主要功能

• 成像功能：对 Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 种参数进行成像分析。测定调节性能量耗散 Y(NPQ)，反映植物光保护能力，测定非调节性能量耗散Y(NO)，反映植物光损伤程度。

• 程序测量功能：可程序测量荧光诱导曲线、快速光曲线和暗弛豫，也可手动测量；在测量过程中能自动分析所有荧光参数的变化趋势

• AOI 功能：可在测量前或测量后任意选择感兴趣的区域（AOI），程序将自动对选择的 AOI 的数据进行变化趋势分析，并在报告文件中显示相关 AOI 的数据。所有报告文件中显示的数据都可导出到 EXCEL 文件中。

• 成像异质性分析功能：对任意参数任意时间的成像，可在图像上任意选取两点，软件自动对两点间的数据进行横向异质性分析，并可导出到 EXCEL 文件中。

• 成像数据范围分析功能：对任意参数任意时间的成像，可分析任意两个荧光数值之间有多少个像素点，多少面积（cm²）。

• 突变株筛选功能：可跟据成像结果快速筛选光合、产氢/油、抗逆（抗盐、抗旱、抗病等）等突变株。

• 微藻毒理研究功能：可同时测量 96 个微藻样品（对照和处理组）的光合活性，软件自动给出处理组样品相对于对照组的光合抑制百分比。

• 吸光系数测量功能：快速测量叶片的吸光系数。吸光系数测量光源： 16 个红光（650 nm）和 16 个近红外（780 nm）LED，用于测量植物叶片或藻类样品 PAR 吸光系数。

测量参数

Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

应用领域

• 光合作用研究：可以在*相同的条件下同时对大量样品进行成像

• 植物病理学：病斑部位（包括肉眼不可见时）成像以及病斑扩散的时空动力学

• 植物胁迫生 理学：肉眼不可见胁迫损伤的早期检测

• 遗传育种：出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株

• 突变株筛选：快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等

• 微藻毒理学：不同毒物浓度多个重复的样品一次测完，软件自动计算抑制比率

• 分子生物学：宏观水平上检测样品的绿色荧光蛋白（GFP）荧光

• 其它多种扩展研究

主要技术参数

MAXI 探头（蓝光版）

• 荧光测量光源： 44 个蓝色 LED，450 nm，测量光强度 0.5 μmol m^-2 s^-1，光化光强度  1900 μmol m^-2 s^-1，饱和脉冲强度 4000 μmol m^-2 s^-1。

• 吸光系数测量光源：16个红光（650 nm）和 16 个近红外（780 nm）LED，用于测量样品 PAR 吸光系数。

• 成像面积：10×13 cm。

• 光强异质性：测量区域光强异质性小于 ±7％。

• 测量参数：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

MAXI 探头（红光版）

• 荧光测量光源： 44 个红色 LED，650 nm，测量光强度 0.5 μmol m^-2 s^-1，光化光强度 1900 μmol m^-2 s^-1，饱和脉冲强度 3700 μmol m^-2 s^-1。

• 吸光系数测量光源：16 个红光（660 nm）和 16 个近红外（780 nm）LED，用于测量样品 PAR 吸光系数。

• 成像面积：11×15 cm。

• 光强异质性：测量区域光强异质性小于 ±7％。

• 测量参数：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

MINI 探头（蓝光版，MINI 探头成像方便野外使用）

• 荧光测量光源：12 个蓝色 LED，460 nm，测量光强度 0.5 μmol m^-2 s^-1，光化光强度 3700 μmol m^-2 s^-1，饱和脉冲强度 8200 μmol m^-2 s^-1。

• 吸光系数测量光源：10 个红光（650 nm）和 10 个近红外（780 nm）LED，用于测量样品 PAR 吸光系数。

• 信号检测：1/3” 数码相机 CCD，640×480 象素，采集速率 30 帧/秒，带选择性锁相放大器。通过千兆以太网（GigE）与电脑数据通讯。

• 成像面积：24×32 mm，6倍放大。

• 测量参数：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

MINI 探头（红光版，MINI 探头成像方便野外使用）

• 荧光测量光源：12 个红色 LED，620 nm，测量光强度 0.5 μmol m^-2 s^-1，光化光强度 2800 μmol m^-2 s^-1，饱和脉冲强度 7700 μmol m^-2 s^-1。

• 吸光系数测量光源：10个红光（650 nm）和 10 个近红外（780 nm）LED，用于测量样品 PAR 吸光系数。

• 信号检测：1/3” 数码相机CCD，640×480 象素，采集速率 30 帧/秒，带选择性锁相放大器。通过千兆以太网（GigE）与电脑数据通讯。

• 成像面积：24×32 mm，6倍放大。

• 测量参数：Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。

选购指南

一、荧光成像 MAXI 版

系统组成：通用型主机，LED 供电单元， LED 光源，CCD，数据线，软件等。

注意：高等植物或真核藻类测量时选择蓝光光源（推荐），蓝藻测量请选择红光光源。

荧光成像 MAXI 版本

二、荧光成像 MINI 版

系统组成：通用型主机（下图中未显示），LED 光源（如下图），CCD，数据线，软件等。

注意：高等植物或真核藻类测量时选择蓝光光源（推荐），蓝藻测量请选择红光光源。

荧光成像 MINI 版

三、荧光成像 MICROSCOPY 版

系统组成：通用型主机，特制显微镜，CCD，数据线，软件等。

荧光成像 MICROSCOPY 版

葡萄叶片 Y(NPQ) 成像	葡萄叶片 PS/50 成像结果	MINI 版本成像结果
显微版本成像结果	软件成像界面	慢速诱导动力学曲线

产地：德国 WALZ

参考文献

数据来源：光合作用文献 Endnote 数据库，更新至 2016 年 9 月，文献数量超过 6000 篇

原始数据来源：Google Scholar

Baek, K., et al. (2016). "DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins." Scientific Reports 6: 30620.

Belbin, F. E., et al. (2016). "Integration of light and circadian signals that regulate chloroplast transcription by a nuclear-encoded sigma factor." New Phytologist: n/a-n/a.

Borlongan, I. A., et al. (2016). "Thermal and PAR effects on the photosynthesis of Eucheuma denticulatum and Kappaphycus striatus (so-called Sacol strain) c*ted in shallow bottom of Bali, Indonesia." Journal of Applied Phycology: 1-10.

Cabral, C., et al. (2016). "Arbuscular mycorrhizal fungi modify nutrient allocation and composition in wheat (Triticum aestivum L.) subjected to heat-stress." Plant and Soil: 1-15.

Chen, D., et al. (2016). "Silicon-moderated K-deficiency-induced leaf chlorosis by decreasing putrescine accumulation in sorghum." Annals of Botany.

Chernysheva, M. G., et al. (2016). "Humic substances alter the uptake and toxicity of nanodiamonds in wheat seedlings." Journal of Soils and Sediments: 1-12.

da Silva, J. M. (2016). "Monitoring Photosynthesis by In Vivo Chlorophyll Fluorescence: Application to High-Throughput Plant Phenotyping."

Gomes, M. P. and P. Juneau (2016). "Oxidative stress in duckweed (Lemna minor L.) induced by glyphosate: Is the mitochondrial electron transport chain a target of this herbicide?" Environmental Pollution.

Gondor, O. K., et al. (2016). "Salicylic Acid and Sodium Salicylate Alleviate Cadmium Toxicity to Different Extents in Maize (Zea mays L.)." PLoS ONE 11(8): e0160157.

Hägele, F., et al. (2016). "Quality Improvement of Fresh-Cut Endive (Cichorium endivia L.) and Recycling of Washing Water by Low-Dose UV-C Irradiation." Food and Bioprocess Technology: 1-12.

Hasan, M. K., et al. (2016). "Glutathione-mediated regulation of nitric oxide, S-nitrosothiol and redox homeostasis confers cadmium tolerance by inducing transcription factors and stress response genes in tomato." Chemosphere 161: 536-545.

Hassler, S., et al. (2016). "Function of the Golgi-located phosphate transporter PHT4;6 is critical for senescence-associated processes in Arabidopsis." Journal of Experimental Botany.

Hu, R., et al. (2016). "Dehydration-responsive features of Atrichum undulatum." Journal of Plant Research: 1-10.

Kakuszi, A., et al. (2016). "Light piping driven photosynthesis in the soil: Low-light adapted active photosynthetic apparatus in the under-soil hypocotyl segments of bean (Phaseolus vulgaris)." Journal of Photochemistry and Photobiology B: Biology 161: 422-429.

Li, L., et al. (2016). "Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana." Journal of Experimental Botany.

Li, L., et al. (2016). "Physiological and biochemical characterization of sheepgrass (Leymus chinensis) reveals insights into photosynthetic apparatus coping with low-phosphate stress conditions." Journal of Plant Biology 59(4): 336-346.

Li, M.-Q., et al. (2016). "Melatonin mediates selenium-induced tolerance to cadmium stress in tomato plants." Journal of pineal research: n/a-n/a.

Li, Q., et al. (2016). "Functional conservation and divergence of GmCHLI genes in polyploid soybean." The Plant Journal: n/a-n/a.

Li, X., et al. (2016). "Melatonin enhances cold tolerance in drought primed wild type and abscisic acid-deficient mutant barley." Journal of pineal research: n/a-n/a.

Li, Z.-X., et al. (2016). "Developmental changes in carbon and nitrogen metabolism affect tea quality in different leaf position." Plant Physiology and Biochemistry 106: 327-335.

Li, Z., et al. (2016). "Evolution of an atypical de-epoxidase for photoprotection in the green lineage." Nature Plants 2: 16140.

Lin, Y.-C., et al. (2016). "Arabidopsis PHOSPHATIDYLGLYCEROPHOSPHATE PHOSPHATASE1 (PGPP1) Involved in Phosphatidylglycerol Biosynthesis and Photosynthetic Function." The Plant Journal: n/a-n/a.

Liu, D., et al. (2016). "Peracetic acid is a suitable disinfectant for recirculating fish-microalgae integrated multi-trophic aquaculture systems." Aquaculture Reports 4: 136-142.

Loro, G., et al. (2016). "Chloroplast-specific in vivo Ca2+ imaging using Yellow Cameleon fluorescent protein sensors reveals organelle-autonomous Ca2+ signatures in the stroma." Plant Physiology.

Nielsen, S. L., et al. (2016). "Copper in the sediment: a major stressor for eelgrass, Zostera marina L." Hydrobiologia: 1-13.

Pell, L., et al. (2016). "Mild disintegration methods of microalgae–bacteria flocs from wastewater treatment." Journal of Applied Phycology: 1-9.

Pompeiano, A., et al. (2016). "Allocation pattern, ion partitioning, and chlorophyll a fluorescence in Arundo donax L. in responses to salinity stress." Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology: 1-10.

Rios, J. A., et al. (2016). "Effects of blast on components of wheat physiology and grain yield as influenced by fungicide treatment and host resistance." Plant Pathology: n/a-n/a.

Schmidt, C., et al. (2016). "Retention of high thermal tolerance in the invasive foraminifera Amphistegina lobifera from the Eastern Mediterranean and the Gulf of Aqaba." Marine Biology 163(11): 228.

Shu, S., et al. (2016). "The role of 24-epibrassinolide in the regulation of photosynthetic characteristics and nitrogen metabolism of tomato seedlings under a combined low temperature and weak light stress." Plant Physiology and Biochemistry 107: 344-353.

Tatagiba, S. D., et al. (2016). "Magnesium decreases leaf scald symptoms on rice leaves and preserves their photosynthetic performance." Plant Physiology and Biochemistry 108: 49-56.

von Sydow, L., et al. (2016). "The PsbY protein of Arabidopsis Photosystem II is important for the redox control of Cytochrome b559." Biochimica et Biophysica Acta (BBA) - Bioenergetics.

Wang, M., et al. (2016). "Submergence Induced Changes of Molecular Species in Membrane Lipids in Arabidopsis thaliana." Plant Diversity.

Wang, P., et al. (2016). "Rapid in-season detection of herbicide resistant Alopecurus myosuroides using a fluorescence imaging sensor." Crop Protection 89: 170-177.

Wang, Y., et al. (2016). "Different responses of photosystem II and antioxidants to drought stress in two contrasting populations of Sour jujube from the Loess Plateau, China." Ecological research: 1-15.

Wang, Y., et al. (2016). "Chlorophyll Fluorescence in Leaves of Ficus tikoua Under Arsenic Stress." Bulletin of Environmental Contamination and Toxicology 97(4): 576-581.

Watanabe, Y., et al. (2016). "Photosynthetic responses of Pyropia yezoensis f. narawaensis (Bangiales, Rhodophyta) to a thermal and PAR gradient vary with the life-history stage." Phycologia 55(6): 665-672.

Xu, Q., et al. (2017). "Volatile organic compounds released from Microcystis flos-aquae under nitrogen sources and their toxic effects on Chlorella vulgaris." Ecotoxicology and Environmental Safety 135: 191-200.

Xu, W., et al. (2016). "Melatonin enhances thermotolerance by promoting cellular protein protection in tomato plants." Journal of pineal research: n/a-n/a.

Zhang, X., et al. (2016). "The miR396b of Poncirus trifoliata functions in cold tolerance by regulating ACC oxidase gene expression and modulating ethylene-polyamine homeostasis." Plant and Cell Physiology.

Zhang, X., et al. (2016). "ER-localized adenine nucleotide transporter ER-ANT1: an integrator of energy and stress signaling in rice." Plant Molecular Biology: 1-15.

Zhang, Y., et al. (2016). "Photosynthetic capacity of the capsule wall and its contribution to carbon fixation and seed yield in castor (Ricinus communis L.)." Acta Physiologiae Plantarum 38(10): 245.