AAT-21140 AAT钙离子指示探针Cal-520,钾盐

背景资料

Cal-520 provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization。 Cal-520 AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention compared to the existing green calcium indicators （such as Fluo-3 AM and Fluo-4 AM）。 Cells expressing a GPCR or calcium channel of interest that signals through calcium can be preloaded with Cal-520 AM which can cross cell membrane。 Once inside the cell， the lipophilic blocking groups of Cal 520 AM are cleaved by esterases， resulting in a negatively charged fluorescent dye that stays inside cells。 Its fluorescence is greatly enhanced upon binding to calcium。 When cells stimulated with agonists， the receptor signals the release of intracellular calcium， which significantly increase the fluorescence of Cal-520。 The characteristics of its long wavelength， high sensitivity， and >100 times fluorescence enhancement， make Cal-520 AM an ideal indicator for the measurement of cellular calcium。 The high S/N ratio and better intracellular retention make the Cal-520 calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists。 Cal-520 sodium or potassium salt is the hydrolyzed salt of Cal-520 AM in cells。 It selectively binds to calcium ion， and has the fluorescence that is strongly dependent on calcium concentration。

产品描述

钙离子指示探针Cal-520， 钾盐产品的光谱学相关参数为Ex（nm）=492/ Em（nm）514；其对应分子量大小为921。08。

产品形式

提供的钙离子指示探针Cal-520， 钾盐产品为固体形式，我们建议将其溶解到DMSO溶液中使用或保存。

保存建议

对于钙离子指示探针Cal-520， 钾盐产品，我们推荐使用常温进行运输。在您收到货物后，我们建议在低于-15℃温度下冷冻保存，要求储存环境干燥并避免光照。产品有效期为12个月。

其他

美国AAT Bioquest Inc。（前身是ABD Bioquest，Inc。）是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域，包括显色、荧光和生物发光技术。AAT Bioquest的产品帮助的科学家和生物医药研究者更好的了解生物化学，免疫学，细胞生物学和分子生物学等领域。作为AAT Bioquest Inc。的中国区域代理，艾美捷科技为中国客户提供光谱学检测技术并应用于生化、生理代谢和细胞分析领域的产品，包括显色，荧光和发光技术等全系列解决方案。